Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,350,162	A→G	L284L (TTG→CTG)	glpQ ←	periplasmic glycerophosphodiester phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,350,162	0	A	G	100.0%	183.8 / NA	52	L284L (TTG→CTG)	glpQ	periplasmic glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/21);  total (31/21)

CAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTC  >  NC_000913/2350036‑2350294                                                                                                                               |                                                                                                                                     ctggAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                          <  2:255459/137‑1 (MQ=255)   ggAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCg                                                                                                                        >  1:163962/1‑139 (MQ=255)         tATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCaa                                                                                                                  >  1:753343/1‑139 (MQ=255)              aGGCAGTTTATCTGACCACACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                             >  2:172806/1‑139 (MQ=255)               ggCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCAt                                                                                                            <  1:624517/139‑1 (MQ=255)                                cACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                           <  2:753343/139‑1 (MQ=255)                                             tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                              >  1:384744/1‑139 (MQ=255)                                             tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                              >  1:391995/1‑139 (MQ=255)                                              gCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCACTCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAcc                                                                             <  2:163962/139‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGATCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  2:263072/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                          >  2:497486/1‑93 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                          <  1:497486/93‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                               <  2:652446/104‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                               >  1:652446/1‑104 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                             <  2:163062/106‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                             >  1:163062/1‑106 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTg                                                                                                         <  1:931834/110‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTg                                                                                                         >  2:931834/1‑110 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCata                                                                                                     >  2:1004432/1‑114 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCata                                                                                                     <  1:1004432/114‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGcc                                                                                              <  2:818650/121‑1 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGcc                                                                                              >  1:818650/1‑121 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  2:91682/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  1:816919/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  1:449766/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  2:330675/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  1:33902/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  2:944211/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  2:207597/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                            >  1:140021/1‑139 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                              >  1:179390/1‑137 (MQ=255)                                               cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                              <  2:179390/137‑1 (MQ=255)                                               cACTA‑CAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                               >  1:21182/1‑103 (MQ=255)                                               cACTA‑CAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                               <  2:21182/103‑1 (MQ=255)                                                aCTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCg                                                                           <  1:172806/139‑1 (MQ=255)                                                     cAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCa                                                                                                       <  1:127189/106‑1 (MQ=255)                                                     cAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCa                                                                                                       >  2:127189/1‑106 (MQ=255)                                                                 ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                          >  2:120893/1‑139 (MQ=255)                                                                 ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                          <  2:816919/139‑1 (MQ=255)                                                                 ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                          <  2:391995/139‑1 (MQ=255)                                                                      cATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCata                                                                                                                                  <  1:91246/62‑1 (MQ=255)                                                                      cATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCata                                                                                                                                  >  2:91246/1‑62 (MQ=255)                                                                         cTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTa                                                  >  2:678624/1‑139 (MQ=255)                                                                              aCCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTaa                                             >  1:799941/1‑139 (MQ=255)                                                                                 aTGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAAccc                                          <  1:944211/139‑1 (MQ=255)                                                                                   gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAg                                        >  1:495498/1‑139 (MQ=255)                                                                                                    tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGttt                       >  2:431271/1‑139 (MQ=255)                                                                                                      cccGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTct                     >  1:75286/1‑139 (MQ=255)                                                                                                          gCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTctgctg                 <  2:449766/139‑1 (MQ=255)                                                                                                           cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAcc                                                                                                              >  2:476206/1‑45 (MQ=255)                                                                                                           cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAcc                                                                                                              <  1:476206/45‑1 (MQ=255)                                                                                                                      cctcAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCa     <  1:91682/139‑1 (MQ=255)                                                                                                                        tcaatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGt   <  1:575925/139‑1 (MQ=255)                                                                                                                         caatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTc  <  2:140021/139‑1 (MQ=255)                                                                                                                         caatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTc  <  1:263072/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                     CAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTC  >  NC_000913/2350036‑2350294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑