Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,398,919 G→A A416V (GCG→GTG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,398,9190GA100.0% 138.0 / NA 43A416V (GCG→GTG) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

ATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA  >  NC_000913/2398784‑2399053
                                                                                                                                       |                                                                                                                                      
aTGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACaa                                                                                                                                     >  2:941943/1‑139 (MQ=255)
             aaCGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCAGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGc                                                                                                                        <  1:154767/139‑1 (MQ=255)
                               ccGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGAACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTgccgc                                                                                                      >  2:680894/1‑139 (MQ=255)
                                 ggTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCa                                                                                                    >  1:805506/1‑139 (MQ=255)
                                  gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc                                                                                                            <  1:650574/130‑1 (MQ=255)
                                  gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc                                                                                                            >  2:650574/1‑130 (MQ=255)
                                  gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAt                                                                                                   >  2:1005186/1‑139 (MQ=255)
                                   tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGt                                                                                                                            >  1:950962/1‑113 (MQ=255)
                                   tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAtt                                                                                                  >  2:665131/1‑139 (MQ=255)
                                   tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAtt                                                                                                  >  2:600395/1‑139 (MQ=255)
                                   tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGACGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGt                                                                                                                            <  2:950962/113‑1 (MQ=255)
                                            aGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt                                                                                         <  2:982682/139‑1 (MQ=255)
                                             gTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTc                                                                                                <  1:428329/131‑1 (MQ=255)
                                             gTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTc                                                                                                >  2:428329/1‑131 (MQ=255)
                                                cACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGTGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc                                                                                     <  2:613725/139‑1 (MQ=255)
                                                            tcatcCAGAGGGGTGTCATCAACGTTGGGAAGAAACAGTGGATTCTTTGTACGTGGACGGGTGTTGAGGGGGGCAAAAATCTGTGAGTCAGCCTCTTTGTGTGCAACCGCTATTTCGCGCGCAGTGCCTTTCACAGCCt                                                                         <  2:1348/139‑1 (MQ=255)
                                                                    aCGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACt                                                                 >  1:962333/1‑139 (MQ=255)
                                                                       ggTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGcc                                                              <  1:633593/139‑1 (MQ=255)
                                                                         tgtCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAg                                                            >  1:676685/1‑139 (MQ=255)
                                                                                  cGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               <  2:773539/95‑1 (MQ=255)
                                                                                  cGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               >  1:773539/1‑95 (MQ=255)
                                                                                     tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt                                                                                         >  2:683024/1‑98 (MQ=255)
                                                                                     tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt                                                                                         <  1:683024/98‑1 (MQ=255)
                                                                                     tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgc                                                >  1:603699/1‑139 (MQ=255)
                                                                                     tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgc                                                >  2:740041/1‑139 (MQ=255)
                                                                                      ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc                                                                                     <  1:896617/101‑1 (MQ=255)
                                                                                      ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc                                                                                     >  2:896617/1‑101 (MQ=255)
                                                                                      ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGGCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTATACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgcc                                               <  1:684359/139‑2 (MQ=255)
                                                                                           caaacaGCGGATGCCTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCTCGTGCTGTGCTGCCGCCATTTCGCTCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCACGCCGGt                                          <  1:680894/139‑1 (MQ=255)
                                                                                                   ggATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTaa                                  <  2:603699/139‑1 (MQ=255)
                                                                                                         ttCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCtt                            <  1:740041/139‑1 (MQ=255)
                                                                                                              aCGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc                       <  2:962333/139‑1 (MQ=255)
                                                                                                                  tGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               >  2:972367/1‑63 (MQ=255)
                                                                                                                  tGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               <  1:972367/63‑1 (MQ=255)
                                                                                                                          gTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc                                                                                                            <  1:920999/42‑1 (MQ=255)
                                                                                                                          gTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc                                                                                                            >  2:920999/1‑42 (MQ=255)
                                                                                                                              aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGt                                    <  2:358748/110‑1 (MQ=255)
                                                                                                                              aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGt                                    >  1:358748/1‑110 (MQ=255)
                                                                                                                                   tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcgc                                                                                              <  1:914375/47‑1 (MQ=255)
                                                                                                                                   tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcgc                                                                                              >  2:914375/1‑47 (MQ=255)
                                                                                                                                   tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCAc                  >  2:215296/1‑123 (MQ=255)
                                                                                                                                   tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCAc                  <  1:215296/123‑1 (MQ=255)
                                                                                                                                   tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTaa  >  2:896390/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA  >  NC_000913/2398784‑2399053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑