Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,420,808	G→A	G63D (GGC→GAC)	yfcG →	GSH‑dependent disulfide bond oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,420,808	0	G	A	100.0%	157.1 / NA	51	G63D (GGC→GAC)	yfcG	GSH‑dependent disulfide bond oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/28);  total (23/28)

AAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACT  >  NC_000913/2420682‑2420943                                                                                                                               |                                                                                                                                        aagCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCt                                                                                                                             >  1:470543/1‑139 (MQ=255)              ttATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAg                                                                                                                >  2:951/1‑139 (MQ=255)                     ttGATTAAGGTCGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTg                                                                                                         <  1:722137/139‑1 (MQ=255)                     ttGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTg                                                                                                         <  2:504922/139‑1 (MQ=255)                     ttGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTg                                                                                                         <  1:686060/139‑1 (MQ=255)                       gATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAGTTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCt                                                                                                                             <  2:823081/117‑1 (MQ=255)                       gATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCt                                                                                                                             >  1:823081/1‑117 (MQ=255)                        aTTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCa                                                                                                      <  1:388082/139‑1 (MQ=255)                                 gACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCa                                                                                                      <  2:287625/130‑1 (MQ=255)                                 gACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCa                                                                                                      >  1:287625/1‑130 (MQ=255)                                 gACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTtgttgt                                                                                             >  1:1073428/1‑139 (MQ=255)                                  aCCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTa                                                                                            >  1:816212/1‑139 (MQ=255)                                  aCCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTa                                                                                            >  2:863426/1‑139 (MQ=255)                                  aCCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTa                                                                                            >  2:1085996/1‑139 (MQ=255)                                      ggggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTg                                                                                        >  2:640160/1‑139 (MQ=255)                                       gggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGa                                                                                                                                   <  2:113290/95‑1 (MQ=255)                                       gggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGa                                                                                                                                   >  1:113290/1‑95 (MQ=255)                                       gggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTgg                                                                                       >  1:956386/1‑139 (MQ=255)                                        ggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCAt                                                                                                     >  2:13125/1‑124 (MQ=255)                                        ggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCAt                                                                                                     <  1:13125/124‑1 (MQ=255)                                        ggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGc                                                                                      >  2:192584/1‑139 (MQ=255)                                               cGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGaaa                                                                               <  1:661497/139‑1 (MQ=255)                                                 gTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAAc                                                                             >  2:260369/1‑139 (MQ=255)                                                 gTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAAc                                                                             >  1:739730/1‑139 (MQ=255)                                                 gTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAAc                                                                             >  1:862420/1‑139 (MQ=255)                                                  tCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACa                                                                            >  2:1017191/1‑139 (MQ=255)                                                     gTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGa                                                                         <  1:611171/139‑1 (MQ=255)                                                      tttCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGAc                                                                        <  1:1017191/139‑1 (MQ=255)                                                               gAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGa                                                               <  1:192584/139‑1 (MQ=255)                                                                aaTTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTc                                                                                                              >  1:798160/1‑91 (MQ=255)                                                                aaTTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTc                                                                                                              <  2:798160/91‑1 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         <  1:631465/139‑1 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         <  1:356399/139‑1 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         <  1:260595/139‑1 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         <  1:260369/139‑1 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         >  2:260595/1‑139 (MQ=255)                                                                                     acaacaaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAgcgcgc                                         <  2:739730/139‑1 (MQ=255)                                                                                         caaAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCgccgcc                                     <  1:1094565/139‑1 (MQ=255)                                                                                            aaTTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCcaca                                  >  1:1066514/1‑139 (MQ=255)                                                                                            aaTTCCGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTGTTGAGTCATGAAACGCGTGAGCGCGCCGCcaca                                  <  2:925349/139‑1 (MQ=255)                                                                                                ccGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTAc                              <  1:863426/139‑1 (MQ=255)                                                                                                 cGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAg                                                                           <  1:242440/93‑1 (MQ=255)                                                                                                 cGGCAATTGTTGATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAg                                                                           >  2:242440/1‑93 (MQ=255)                                                                                                       ttgttgATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGtt                       <  2:956386/139‑1 (MQ=255)                                                                                                        tgttgATCATTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTa                      <  1:1085996/139‑1 (MQ=255)                                                                                                                aTTCTCCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCa              <  2:816212/139‑1 (MQ=255)                                                                                                                  tctcCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTgg                                                                                                           <  2:378061/44‑1 (MQ=255)                                                                                                                  tctcCTGCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTgg                                                                                                           >  1:378061/1‑44 (MQ=255)                                                                                                                        gCCGATGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAACCGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCg      >  2:163158/1‑139 (MQ=255)                                                                                                                            aTGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACt  <  2:1066514/139‑1 (MQ=255)                                                                                                                            aTGACGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACt  <  1:1051717/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        AAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAGCCTCTTTGAGTCTGGTGCCATTTTGTTGTATCTGGCTGAGAAAACAGGACTCTTTTTGAGTCATGAAACGCGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACT  >  NC_000913/2420682‑2420943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑