Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,434,929	C→T	A236A (GCG→GCA)	truA ←	tRNA pseudouridine(38‑40) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,929	0	C	T	100.0%	173.5 / NA	54	A236A (GCG→GCA)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/24);  total (30/24)

GTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAAT  >  NC_000913/2434823‑2435057                                                                                                           |                                                                                                                                 gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAActc                                                                                                  >  2:521032/1‑139 (MQ=255)   tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                                                <  1:521032/139‑1 (MQ=255)      tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTa                                                                                             >  2:170039/1‑139 (MQ=255)      tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTa                                                                                             >  1:370438/1‑139 (MQ=255)                        cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGtt                                                                                                                         <  2:912361/93‑1 (MQ=255)                        cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGtt                                                                                                                         >  2:292039/1‑93 (MQ=255)                        cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGtt                                                                                                                         >  1:912361/1‑93 (MQ=255)                        cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGtt                                                                                                                         <  1:292039/93‑1 (MQ=255)                                     ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc                                                              >  2:1054302/1‑139 (MQ=255)                                                tACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                                                   >  1:77910/1‑139 (MQ=255)                                                    ggTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                                               <  2:674412/139‑1 (MQ=255)                                                     gTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCaa                                                                                                     >  2:87170/1‑84 (MQ=255)                                                     gTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCaa                                                                                                     <  1:87170/84‑1 (MQ=255)                                                     gTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                                              >  1:1086346/1‑139 (MQ=255)                                                      tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             >  2:320771/1‑139 (MQ=255)                                                      tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             <  2:370438/139‑1 (MQ=255)                                                      tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             >  1:733360/1‑139 (MQ=255)                                                      tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             >  1:889056/1‑139 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCaa                                                                                                     <  2:510135/74‑1 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCaa                                                                                                     >  1:510135/1‑74 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                                                    >  2:735606/1‑123 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                                                    <  1:735606/123‑1 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc                                    <  1:1054302/139‑1 (MQ=255)                                                               tCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc                                    <  2:889056/139‑1 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                                           <  2:1011971/127‑1 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                                           >  1:1011971/1‑127 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg                                  <  2:1102498/136‑1 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg                                  >  1:1102498/1‑136 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               >  1:217830/1‑139 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               >  2:902783/1‑139 (MQ=255)                                                                    cgcgACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               >  2:182253/1‑139 (MQ=255)                                                                       gACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCata                            <  1:249384/139‑1 (MQ=255)                                                                         ccAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATatg                          <  2:217830/139‑1 (MQ=255)                                                                              tACAGCCCTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTa                     <  1:938589/139‑1 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                                                      <  2:28833/53‑1 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                                                      >  1:28833/1‑53 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaaa                >  2:354727/1‑139 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaaa                >  2:446510/1‑139 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACa                   >  1:472460/1‑136 (MQ=255)                                                                                   cccTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACa                   <  2:472460/136‑1 (MQ=255)                                                                                     cTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             >  1:624254/1‑108 (MQ=255)                                                                                     cTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                                             <  2:624254/108‑1 (MQ=255)                                                                                     cTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               <  2:301094/122‑1 (MQ=255)                                                                                     cTTCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               >  1:301094/1‑122 (MQ=255)                                                                                      ttCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCa             >  2:471138/1‑139 (MQ=255)                                                                                      ttCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCa             <  1:170039/139‑1 (MQ=255)                                                                                       tCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc                                                                  <  1:656202/85‑1 (MQ=255)                                                                                       tCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCAt            >  1:715147/1‑139 (MQ=255)                                                                                       tCCGCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGCCAc                                                                  >  2:656202/1‑85 (MQ=255)                                                                                          gCTTTTGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCg         <  1:182253/139‑1 (MQ=255)                                                                                             tttGCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCtt      >  2:51713/1‑139 (MQ=255)                                                                                                gCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               <  1:911574/111‑1 (MQ=255)                                                                                                gCCGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc                               >  2:911574/1‑111 (MQ=255)                                                                                                 ccGTTGCTGCTGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAat  <  2:589862/139‑1 (MQ=255)                                                                                                           |                                                                                                                                 GTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAAT  >  NC_000913/2434823‑2435057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑