Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,238	C→T	G437G (GGC→GGT)	bamA →	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,238	0	C	T	100.0%	106.9 / NA	35	G437G (GGC→GGT)	bamA	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

CTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTA  >  NC_000913/199117‑199357                                                                                                                          |                                                                                                                        cTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTgg                                                                                                        <  1:1030387/139‑1 (MQ=255)   gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGgtg                                                                                                      >  1:276624/1‑139 (MQ=255)   gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGgtg                                                                                                      <  1:908547/139‑1 (MQ=255)        taccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTgcag                                                                                                 <  2:934497/139‑1 (MQ=255)              taccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGAt                                                                                           >  2:252871/1‑139 (MQ=255)                    aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                                                     <  1:763992/139‑1 (MQ=255)                     cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    <  2:873076/139‑1 (MQ=255)                     cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    <  2:1066900/139‑1 (MQ=255)                      gtgtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTTAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                                                   <  2:503846/139‑1 (MQ=255)                          tCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                                               >  2:764976/1‑139 (MQ=255)                          tCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGGGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                                               <  2:269713/139‑1 (MQ=255)                           ccGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTa                                                                              >  2:1069851/1‑139 (MQ=255)                            cGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTAc                                                                             <  2:987822/139‑1 (MQ=255)                            cGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTAc                                                                             <  2:276624/139‑1 (MQ=255)                                   cccGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTAt                                                                      >  2:276559/1‑139 (MQ=255)                                        aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    >  1:951038/1‑120 (MQ=255)                                        aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    <  2:951038/120‑1 (MQ=255)                                        aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTa                                                                       <  1:473405/133‑1 (MQ=255)                                        aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTa                                                                       >  2:473405/1‑133 (MQ=255)                                                       tcTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTCCTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACCGGTTATGCTGTTGGTATCAACGGGAc                                                  >  2:536816/1‑139 (MQ=255)                                                       tcTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCa                                                                                                              >  1:635907/1‑79 (MQ=255)                                                       tcTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGAc                                                  >  1:1075544/1‑139 (MQ=255)                                                       tcTACAACGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGATAGTGGTGTGAGCTTCCa                                                                                                              <  2:635907/79‑1 (MQ=255)                                                                   aaGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTa                                      >  1:426032/1‑139 (MQ=255)                                                                     gagCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAG‑‑‑‑CAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc                                <  2:419116/139‑1 (MQ=255)                                                                        cgcAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGa                                 >  1:1051496/1‑139 (MQ=255)                                                                         gcAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc                                <  2:1026411/139‑1 (MQ=255)                                                                         gcAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc                                >  2:881728/1‑139 (MQ=255)                                                                          cAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    >  2:270522/1‑86 (MQ=255)                                                                          cAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                                                    <  1:270522/86‑1 (MQ=255)                                                                                                   aTTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTAc                                     >  2:450313/1‑108 (MQ=255)                                                                                                   aTTGGTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTAc                                     <  1:450313/108‑1 (MQ=255)                                                                                                      ggTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg                                          <  1:1026288/100‑1 (MQ=255)                                                                                                      ggTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg                                          >  2:1026288/1‑100 (MQ=255)                                                                                                       gTTACGGTACTGAAAGTGGTGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTa  <  1:881728/139‑1 (MQ=255)                                                                                                                          |                                                                                                                        CTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTA  >  NC_000913/199117‑199357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑