Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,798	A→G	intergenic (+152/‑214)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,798	0	A	G	100.0%	141.8 / NA	40	intergenic (+152/‑214)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

CAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTT  >  NC_000913/2462681‑2462929                                                                                                                      |                                                                                                                                    cAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCat                                                                                                                >  1:587196/1‑139 (MQ=255)   ggTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCatat                                                                                                              >  2:432592/1‑139 (MQ=255)   ggTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCatat                                                                                                              >  1:721080/1‑139 (MQ=255)      gACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGATTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAATGGAAAGGCATAtctt                                                                                                           >  2:439445/1‑139 (MQ=255)      gACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATAtttt                                                                                                           >  1:1015947/1‑139 (MQ=255)         tttAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAg                                                                                                        >  2:338092/1‑139 (MQ=255)             aCTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGt                                                                                                    <  2:1034752/139‑1 (MQ=255)                 ccccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTccg                                                                                                >  1:981921/1‑137 (MQ=255)                  cccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTccgc                                                                                               >  1:883207/1‑136 (MQ=255)                      cTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                           <  2:1050738/139‑1 (MQ=255)                        tCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTcc                                                                                         >  1:770202/1‑139 (MQ=255)                              aaaGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                   <  2:770202/139‑1 (MQ=255)                                     tgtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTtctc                                                                                    <  1:368767/131‑1 (MQ=255)                                     tgtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTtctc                                                                                    >  2:368767/1‑131 (MQ=255)                                      gtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGttt                                                                           >  2:617388/1‑139 (MQ=255)                                         cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACt                                                                        >  1:680011/1‑139 (MQ=255)                                         cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACt                                                                        >  1:22473/1‑139 (MQ=255)                                         cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGATTACt                                                                        >  1:878185/1‑139 (MQ=255)                                            aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAag                                                                                >  1:967183/1‑128 (MQ=255)                                            aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAag                                                                                <  2:967183/128‑1 (MQ=255)                                             ttGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTc                                                                    <  2:1015947/139‑1 (MQ=255)                                                cTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                 <  1:338092/139‑1 (MQ=255)                                                      tcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTcc                                                           >  1:11899/1‑139 (MQ=255)                                                        ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                         >  2:377230/1‑139 (MQ=255)                                                        ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                         >  2:195452/1‑139 (MQ=255)                                                          gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGa                                                       >  1:987215/1‑139 (MQ=255)                                                           aGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAt                                                      <  2:414870/139‑1 (MQ=255)                                                                  cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCTCTCGTCCTTCTCa                                                                                   <  2:745512/103‑1 (MQ=255)                                                                  cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                               <  1:432592/139‑1 (MQ=255)                                                                  cAAACAGCCCGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                   >  1:745512/1‑103 (MQ=255)                                                                    aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                             <  1:377230/139‑1 (MQ=255)                                                                    aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                             <  2:11899/139‑1 (MQ=255)                                                                            gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTa                                    <  2:22473/139‑1 (MQ=255)                                                                             ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTAt                                   >  1:938184/1‑139 (MQ=255)                                                                                             tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                               <  2:177865/127‑1 (MQ=255)                                                                                             tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                               >  1:177865/1‑127 (MQ=255)                                                                                              cAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                  <  2:721080/139‑1 (MQ=255)                                                                                                            gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg                             <  2:295896/114‑1 (MQ=255)                                                                                                            gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg                             >  1:295896/1‑114 (MQ=255)                                                                                                              aTCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt  <  2:587196/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                    CAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTT  >  NC_000913/2462681‑2462929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑