Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,519,189 T→C I126T (ATT→ACT)  yfeD → DUF1323 family putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,1890TC100.0% 151.4 / NA 43I126T (ATT→ACT) yfeDDUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/19);  total (24/19)

GCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGG  >  NC_000913/2519056‑2519320
                                                                                                                                     |                                                                                                                                   
gCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACg                                                                                                                                >  2:1078288/1‑139 (MQ=255)
            cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCataaa                                                                                                                    >  2:828137/1‑139 (MQ=255)
                       gTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         <  1:79753/139‑1 (MQ=255)
                          tCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAgg                                                                                                      <  1:828137/139‑1 (MQ=255)
                                    atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        <  1:1090255/127‑1 (MQ=255)
                                    atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        >  2:1090255/1‑127 (MQ=255)
                                              tagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTa                                                                                  >  1:387254/1‑139 (MQ=255)
                                                     tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                           >  2:1102930/1‑139 (MQ=255)
                                                       tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                         >  2:127062/1‑139 (MQ=255)
                                                        gacagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTaca                                                                        >  1:562465/1‑139 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGa                                                                                                                                  >  1:730752/1‑79 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGa                                                                                                                                  <  2:730752/79‑1 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACgg                                                                                                          <  1:920503/103‑1 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACgg                                                                                                          >  2:920503/1‑103 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         <  1:624883/104‑1 (MQ=255)
                                                          cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGc                                                                                                         >  2:624883/1‑104 (MQ=255)
                                                             acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    >  1:588395/1‑122 (MQ=255)
                                                             acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    >  1:939546/1‑122 (MQ=255)
                                                             acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    <  2:588395/122‑1 (MQ=255)
                                                             acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    <  2:939546/122‑1 (MQ=255)
                                                                ttGAGGAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGt                                                                                     <  2:122756/118‑1 (MQ=255)
                                                                ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGccat                                                                                     >  1:122756/1‑116 (MQ=255)
                                                                   aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg                                                                      <  2:142225/130‑1 (MQ=255)
                                                                   aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg                                                                      >  1:142225/1‑130 (MQ=255)
                                                                             aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                           >  1:779064/1‑115 (MQ=255)
                                                                             aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                           <  2:779064/115‑1 (MQ=255)
                                                                             aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                   >  2:970948/1‑139 (MQ=255)
                                                                                cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                <  1:970948/139‑1 (MQ=255)
                                                                                     aaTTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAg                                           <  2:562465/139‑1 (MQ=255)
                                                                                                 gTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCa                               >  1:605557/1‑139 (MQ=255)
                                                                                                       gAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                          >  2:117105/1‑90 (MQ=255)
                                                                                                       gAATTCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                          <  1:117105/90‑1 (MQ=255)
                                                                                                           tCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        >  2:245966/1‑56 (MQ=255)
                                                                                                           tCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCa                                                                                                        <  1:245966/56‑1 (MQ=255)
                                                                                                           tCGCAATTTCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                     >  2:1043809/1‑139 (MQ=255)
                                                                                                                 tttCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    <  2:281626/70‑1 (MQ=255)
                                                                                                                 tttCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                    >  1:281626/1‑70 (MQ=255)
                                                                                                                  ttCCTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGc              >  2:448570/1‑139 (MQ=255)
                                                                                                                     cTCGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg           <  1:448570/139‑1 (MQ=255)
                                                                                                                       cGCCCGTCTCGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGTTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt         >  1:98649/1‑139 (MQ=255)
                                                                                                                             tctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg   >  1:996438/1‑139 (MQ=255)
                                                                                                                              ctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCgg  <  2:387254/139‑1 (MQ=255)
                                                                                                                              ctcGATACTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCgg  <  1:1043809/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGG  >  NC_000913/2519056‑2519320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑