Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,589,535 C→T I647I (ATC→ATT acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,589,5350CT100.0% 84.4 / NA 27I647I (ATC→ATTacrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

AACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAG  >  NC_000913/2589404‑2589659
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aacaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAATGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTg                                                                                                                       <  2:141937/139‑1 (MQ=255)
             tgtTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGTCAAAACGTGGCGCGAATGTTTATCCGCCTTAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTTATCGTGCAACGAAGGCgat                                                                                                          >  2:21262/1‑137 (MQ=255)
               tttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa                                                                                                                <  2:70303/131‑1 (MQ=255)
               tttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa                                                                                                                >  1:70303/1‑131 (MQ=255)
                       cGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAAtt                                                                                                >  1:148498/1‑139 (MQ=255)
                            gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGa                                                                                                                            >  2:341227/1‑106 (MQ=255)
                            gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGa                                                                                                                            <  1:341227/106‑1 (MQ=255)
                                 ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                      >  2:1095498/1‑139 (MQ=255)
                                   cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgc                                                                                    >  2:270833/1‑139 (MQ=255)
                                              aCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCcag                                                                         >  2:594255/1‑139 (MQ=255)
                                                           ggCGCGAATGTTTATATGCCTGAACGACTGGAGCGAACGCGAGAGGAAGACCGGCACCTCGTTTGCCATTATTGAGCTTGCACCGAAGGCGGTTAGCCAACTGAAAGAAGCTCGCGCTTTCGCCAGTAGCCGGCCAGCa                                                            >  2:683551/1‑139 (MQ=255)
                                                                             ccTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGt                                          >  2:615567/1‑139 (MQ=255)
                                                                               tGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTc                                        >  2:659963/1‑139 (MQ=255)
                                                                               tGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTc                                        >  1:1114505/1‑139 (MQ=255)
                                                                                 aaaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg                                      <  1:1095498/139‑1 (MQ=255)
                                                                                  aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc                                     <  1:270833/139‑1 (MQ=255)
                                                                                  aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc                                     <  1:594255/139‑1 (MQ=255)
                                                                                  aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc                                     <  2:1114505/139‑1 (MQ=255)
                                                                                                               ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTa                                                        >  2:4189/1‑91 (MQ=255)
                                                                                                               ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTa                                                        <  1:4189/91‑1 (MQ=255)
                                                                                                                gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTaa                                                                                              >  1:1035166/1‑52 (MQ=255)
                                                                                                                gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTaa                                                                                              <  2:1035166/52‑1 (MQ=255)
                                                                                                                 cACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt                                                                                 >  2:689017/1‑64 (MQ=255)
                                                                                                                 cACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt                                                                                 <  1:689017/64‑1 (MQ=255)
                                                                                                                    cTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                      >  1:116184/1‑56 (MQ=255)
                                                                                                                    cTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                      <  2:116184/56‑1 (MQ=255)
                                                                                                                     tCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAg  <  1:615567/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                            
AACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAG  >  NC_000913/2589404‑2589659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: