Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,589,535 | C→T | I647I (ATC→ATT) | acrD → | aminoglycoside/multidrug efflux system |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,589,535 | 0 | C | T | 100.0% | 84.4 / NA | 27 | I647I (ATC→ATT) | acrD | aminoglycoside/multidrug efflux system |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/12); total (15/12) |
AACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAG > NC_000913/2589404‑2589659 | aacaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAATGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTg < 2:141937/139‑1 (MQ=255) tgtTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGTCAAAACGTGGCGCGAATGTTTATCCGCCTTAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTTATCGTGCAACGAAGGCgat > 2:21262/1‑137 (MQ=255) tttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa < 2:70303/131‑1 (MQ=255) tttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa > 1:70303/1‑131 (MQ=255) cGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAAtt > 1:148498/1‑139 (MQ=255) gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGa > 2:341227/1‑106 (MQ=255) gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGa < 1:341227/106‑1 (MQ=255) ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc > 2:1095498/1‑139 (MQ=255) cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgc > 2:270833/1‑139 (MQ=255) aCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCcag > 2:594255/1‑139 (MQ=255) ggCGCGAATGTTTATATGCCTGAACGACTGGAGCGAACGCGAGAGGAAGACCGGCACCTCGTTTGCCATTATTGAGCTTGCACCGAAGGCGGTTAGCCAACTGAAAGAAGCTCGCGCTTTCGCCAGTAGCCGGCCAGCa > 2:683551/1‑139 (MQ=255) ccTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGt > 2:615567/1‑139 (MQ=255) tGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTc > 2:659963/1‑139 (MQ=255) tGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTc > 1:1114505/1‑139 (MQ=255) aaaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg < 1:1095498/139‑1 (MQ=255) aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc < 1:270833/139‑1 (MQ=255) aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc < 1:594255/139‑1 (MQ=255) aaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGc < 2:1114505/139‑1 (MQ=255) ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTa > 2:4189/1‑91 (MQ=255) ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTa < 1:4189/91‑1 (MQ=255) gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTaa > 1:1035166/1‑52 (MQ=255) gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTaa < 2:1035166/52‑1 (MQ=255) cACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt > 2:689017/1‑64 (MQ=255) cACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt < 1:689017/64‑1 (MQ=255) cTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc > 1:116184/1‑56 (MQ=255) cTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc < 2:116184/56‑1 (MQ=255) tCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAg < 1:615567/139‑1 (MQ=255) | AACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAG > NC_000913/2589404‑2589659 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |