Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,591,603 G→A *119* (TAG→TAA yffB → putative ArsC family reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,591,6030GA100.0% 148.1 / NA 47*119* (TAG→TAAyffBputative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)

CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTG  >  NC_000913/2591467‑2591727
                                                                                                                                        |                                                                                                                            
ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATc                                                                                                                            >  2:428100/1‑139 (MQ=255)
ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATc                                                                                                                            >  2:603215/1‑139 (MQ=255)
ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATc                                                                                                                            >  2:561081/1‑139 (MQ=255)
           tctGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGcc                                                                                                                 <  1:603215/139‑1 (MQ=255)
                   ggcATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAtt                                                                                                         <  1:1105839/139‑1 (MQ=255)
                          aTGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGa                                                                                                  <  1:11988/139‑1 (MQ=255)
                                 aaaTGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaaca                                                                                           >  1:27105/1‑139 (MQ=255)
                                     gCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCtt                                                                                       >  1:141080/1‑139 (MQ=255)
                                        tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAtt                                                                                    >  1:377317/1‑139 (MQ=255)
                                        tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGAAACAACAGCTTAtt                                                                                    >  1:356876/1‑139 (MQ=255)
                                                       tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTTGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCTGCCCTTCCCTg                                                                     >  2:136716/1‑139 (MQ=255)
                                                          aTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGaca                                                                                                >  1:641735/1‑109 (MQ=255)
                                                          aTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                  <  1:136716/139‑1 (MQ=255)
                                                          aTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAAGCTATGTCGTGCCCGGTTATTGAGCTGaca                                                                                                <  2:641735/109‑1 (MQ=255)
                                                                ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAtt                                                                                                         <  2:647929/94‑1 (MQ=255)
                                                                ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAtt                                                                                                         >  1:647929/1‑94 (MQ=255)
                                                                  gCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                          <  2:377317/139‑1 (MQ=255)
                                                                   cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTgatgat                                                         >  1:1082712/1‑139 (MQ=255)
                                                                      gCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccg                                                                               <  2:1001461/114‑1 (MQ=255)
                                                                      gCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccg                                                                               >  1:1001461/1‑114 (MQ=255)
                                                                      gCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCa                                                      <  1:561081/139‑1 (MQ=255)
                                                                       cccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAg                                                     >  1:1059652/1‑139 (MQ=255)
                                                                        ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAgg                                                    >  1:563776/1‑139 (MQ=255)
                                                                             aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                             >  2:309955/1‑109 (MQ=255)
                                                                             aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                             <  1:309955/109‑1 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                          <  1:630352/116‑1 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                          <  1:295399/116‑1 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                          >  2:630352/1‑116 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                          >  2:295399/1‑116 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCt                                                               <  1:696776/111‑1 (MQ=255)
                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCt                                                               >  2:696776/1‑111 (MQ=255)
                                                                                          tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                       <  2:460558/102‑1 (MQ=255)
                                                                                          tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                       >  1:460558/1‑102 (MQ=255)
                                                                                           gggTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCGGGCTTTGttgatt                                 >  2:94991/1‑139 (MQ=255)
                                                                                             gTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgattga                               <  2:356876/139‑1 (MQ=255)
                                                                                              tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGaa                              >  1:348633/1‑139 (MQ=255)
                                                                                                      aTTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAgg                                                    <  2:474281/109‑1 (MQ=255)
                                                                                                      aTTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAgg                                                    >  1:474281/1‑109 (MQ=255)
                                                                                                            gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGAt                <  1:428100/139‑1 (MQ=255)
                                                                                                                  aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCtt                                                                                       >  2:526212/1‑62 (MQ=255)
                                                                                                                  aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCtt                                                                                       <  1:526212/62‑1 (MQ=255)
                                                                                                                  aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCt                                          <  2:811619/107‑1 (MQ=255)
                                                                                                                  aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCt                                          >  1:811619/1‑107 (MQ=255)
                                                                                                                          ttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg  <  2:563776/139‑1 (MQ=255)
                                                                                                                          ttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg  <  2:348633/139‑1 (MQ=255)
                                                                                                                                    tgtAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGaa                              <  2:257363/101‑1 (MQ=255)
                                                                                                                                    tgtAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGaa                              >  1:257363/1‑101 (MQ=255)
                                                                                                                                        |                                                                                                                            
CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTG  >  NC_000913/2591467‑2591727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: