Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,015	G→A	*427* (TAG→TAA)	gabT →	4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,015	0	G	A	100.0%	146.4 / NA	46	*427* (TAG→TAA)	gabT	4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

AAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793882‑2794141                                                                                                                                      |                                                                                                                               aaGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAAcgccg                                                                                                                           <  1:517396/139‑1 (MQ=255)               ctctcCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAg                                                                                                             >  2:952545/1‑139 (MQ=255)                                 tACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTg                                                                                           <  2:966538/139‑1 (MQ=255)                                   cAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTc                                                                                         <  2:912947/139‑1 (MQ=255)                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       >  1:140244/1‑136 (MQ=255)                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       <  2:781884/136‑1 (MQ=255)                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       <  2:140244/136‑1 (MQ=255)                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       >  1:781884/1‑136 (MQ=255)                                         gctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCt                                                                                   >  2:9804/1‑139 (MQ=255)                                             cgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACgg                                                                               <  1:9804/139‑1 (MQ=255)                                             cgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACgg                                                                               >  1:883352/1‑139 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       <  2:121370/116‑1 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCg                                                                                       >  1:121370/1‑116 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                                           <  2:497786/128‑1 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                                           >  1:497786/1‑128 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     >  1:852075/1‑134 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     <  2:852075/134‑1 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:585431/1‑139 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:516423/1‑139 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  1:606077/1‑139 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:316493/1‑139 (MQ=255)                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:241148/1‑139 (MQ=255)                                                                                  tCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                          <  1:516423/139‑1 (MQ=255)                                                                                    cGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACa                                        <  2:606077/139‑1 (MQ=255)                                                                                          ggTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                                   <  2:190984/74‑1 (MQ=255)                                                                                          ggTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                                   >  1:190984/1‑74 (MQ=255)                                                                                                gagaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCg                                           >  1:396725/1‑124 (MQ=255)                                                                                                gagaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCg                                           <  2:396725/124‑1 (MQ=255)                                                                                                 agaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGaa                           <  1:316493/139‑1 (MQ=255)                                                                                                      atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:291912/1‑97 (MQ=255)                                                                                                      atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                <  1:291912/97‑1 (MQ=255)                                                                                                      atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      >  1:942863/1‑139 (MQ=255)                                                                                                        caGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                    <  1:952545/139‑1 (MQ=255)                                                                                                               tgtTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                    >  2:345190/1‑68 (MQ=255)                                                                                                               tgtTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                                    <  1:345190/68‑1 (MQ=255)                                                                                                                gtTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTAt            <  2:883352/139‑1 (MQ=255)                                                                                                                 ttttGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGa                                                                 >  1:1043267/1‑85 (MQ=255)                                                                                                                 ttttGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGa                                                                 <  2:1043267/85‑1 (MQ=255)                                                                                                                     gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg       <  1:241148/139‑1 (MQ=255)                                                                                                                         aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                     <  2:731469/105‑1 (MQ=255)                                                                                                                         aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                     >  1:731469/1‑105 (MQ=255)                                                                                                                         aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                          >  2:849261/1‑116 (MQ=255)                                                                                                                         aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                          <  1:849261/116‑1 (MQ=255)                                                                                                                          ggCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg  >  2:953125/1‑139 (MQ=255)                                                                                                                           gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                     <  2:695773/103‑1 (MQ=255)                                                                                                                           gCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                     >  1:695773/1‑103 (MQ=255)                                                                                                                                      |                                                                                                                               AAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793882‑2794141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑