| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 30,616 | T→C | P322P (CCT→CCC) | carA → | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 30,616 | 0 | T | C | 97.3% | 126.8 / ‑4.9 | 37 | P322P (CCT→CCC) | carA | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (18/18); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGT > NC_000913/30500‑30746 | gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa > 1:831951/1‑139 (MQ=255)gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa > 1:513668/1‑139 (MQ=255) aTTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTc > 2:780766/1‑139 (MQ=255) ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa < 2:513668/139‑1 (MQ=255) ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa < 1:832911/139‑1 (MQ=255) cggcggCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTa < 1:1089036/139‑1 (MQ=255) aaCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCa < 2:831951/139‑1 (MQ=255) gATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATa < 1:345806/139‑1 (MQ=255) tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGtt > 2:280920/1‑112 (MQ=255) tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGtt < 1:280920/112‑1 (MQ=255) tgGAG‑AAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCg > 2:819768/1‑139 (MQ=255) ggAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCg > 1:607648/1‑139 (MQ=255) gagaaaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGc < 1:819768/136‑1 (MQ=255) aaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCTTTCa < 1:424937/139‑1 (MQ=255) tGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGt > 1:774671/1‑89 (MQ=255) tGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGt < 2:774671/89‑1 (MQ=255) tCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCAt < 2:235082/68‑1 (MQ=255) tCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCAt > 1:235082/1‑68 (MQ=255) aCCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAcc > 1:433618/1‑139 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtg > 1:439262/1‑56 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtg < 2:439262/56‑1 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc > 1:254402/1‑58 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc < 2:254402/58‑1 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa < 1:866871/59‑1 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa > 2:866871/1‑59 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCCGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt > 1:2085/1‑139 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCCGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt < 2:2085/139‑1 (MQ=255) cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGa > 1:497742/1‑139 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCAc > 2:481242/1‑55 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCAc < 1:481242/55‑1 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc < 2:234326/139‑1 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc < 2:607648/139‑1 (MQ=255) cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa > 1:791281/1‑55 (MQ=255) cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa < 2:791281/55‑1 (MQ=255) gACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg > 1:191879/1‑139 (MQ=255) gACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg > 1:779053/1‑139 (MQ=255) aCATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCgt < 2:963960/139‑1 (MQ=255) | GTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGT > NC_000913/30500‑30746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |