Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,659	A→G	C20R (TGT→CGT)	pepD ←	aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,659	0	A	G	100.0%	239.4 / NA	66	C20R (TGT→CGT)	pepD	aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793                                                                                                                                  |                                                                                                                                       cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  2:81727/139‑1 (MQ=255) cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  2:1060269/1‑139 (MQ=255)      gCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACTGGTGAGGAATAGAACGGATTTTGGCaaaaa                                                                                                                          >  1:973749/1‑139 (MQ=255)            ttACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGATGGGTGAGGAATAGAACGGAtatttttgccaaaat                                                                                                                       >  1:1084746/1‑125 (MQ=255)            ttACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGAtatttttgccaaaat                                                                                                                       <  2:1084746/136‑12 (MQ=255)                gAATCAGGATACTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATTTCCCACAg                                                                                                                <  1:723094/139‑1 (MQ=255)                   tCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  1:957999/139‑1 (MQ=255)                    cAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGc                                                                                                            <  2:467149/139‑1 (MQ=255)                    cAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCAACAGCGGc                                                                                                            >  2:631306/1‑139 (MQ=255)                     aGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           >  1:1044426/1‑139 (MQ=255)                     aGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  2:538806/139‑1 (MQ=255)                     aGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  2:440194/139‑1 (MQ=255)                             acctacctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGAt                                                                                                   <  1:1060269/139‑1 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  2:802912/1‑139 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  1:957171/1‑139 (MQ=255)                                      aTCGCGTTCGACATGGAGACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCACGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  1:694961/1‑139 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATcc                                                                                                                     <  1:568253/112‑1 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATcc                                                                                                                     >  2:568253/1‑112 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAg                                                                                        >  1:941604/1‑139 (MQ=255)                                            ttCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCa                                                                                    <  1:348471/139‑1 (MQ=255)                                            ttCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCa                                                                                    <  2:1044426/139‑1 (MQ=255)                                                     gAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTa                                                                           <  2:184729/139‑1 (MQ=255)                                                          cTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGtct                                                                      <  2:573304/139‑1 (MQ=255)                                                           tttCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGtctc                                                                     >  2:885610/1‑139 (MQ=255)                                                                    tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGAt                                                            >  1:683848/1‑139 (MQ=255)                                                                          accaacAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCg                                                      <  1:802912/139‑1 (MQ=255)                                                                                   gTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCaa                                                                                                                             >  2:471732/1‑59 (MQ=255)                                                                                   gTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCaa                                                                                                                             <  1:471732/59‑1 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTgg                                                                                                       >  1:15703/1‑79 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTgg                                                                                                       <  2:15703/79‑1 (MQ=255)                                                                                      ttCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  2:661740/1‑69 (MQ=255)                                                                                      ttCAGCCAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:661740/69‑1 (MQ=255)                                                                                        cAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        >  2:536652/1‑139 (MQ=255)                                                                                        cAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  1:885610/139‑1 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  2:270220/1‑139 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  1:530521/1‑139 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCa                                                    >  1:1062875/1‑125 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCa                                                    <  2:1062875/125‑1 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTca                                  >  2:1043625/1‑139 (MQ=255)                                                                                               ttGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTcac                                 <  1:356387/139‑1 (MQ=255)                                                                                                   tctTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  2:937102/1‑109 (MQ=255)                                                                                                   tctTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:937102/109‑1 (MQ=255)                                                                                                     ttCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGtt                           <  1:270220/139‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgag                                                                                            >  1:861511/1‑64 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgag                                                                                            <  2:861511/64‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 >  2:474858/1‑139 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 >  1:506975/1‑139 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCcac                                                                                                                  >  2:310414/1‑40 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCcac                                                                                                                  <  1:310414/40‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  2:44867/47‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           >  1:44867/1‑47 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            >  1:816174/1‑110 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            <  2:816174/110‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGGGATAATTgaga                                                                                           <  2:435579/60‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGGGATAATTgaga                                                                                           >  1:435579/1‑60 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  2:530521/139‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  2:683848/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  2:756589/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:474858/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:348580/139‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:75599/40‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  1:75599/1‑40 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGa  >  1:477793/1‑139 (MQ=255)                                                                                                                              gAACGGAATTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGGCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATCTTGTCAACACAAGa  >  1:680280/1‑139 (MQ=255)                                                                                                                               aaCGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:1103934/81‑1 (MQ=255)                                                                                                                               aaCGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  2:1103934/1‑81 (MQ=255)                                                                                                                                  |                                                                                                                                       CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑