Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,893,928 G→A *424* (TAG→TAA
A4T (GCC→ACC) 
ygcN →
ygcO →
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,893,9280GA100.0% 139.6 / NA 44*424* (TAG→TAA
A4T (GCC→ACC) 
ygcN
ygcO
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

ATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAA  >  NC_000913/2893795‑2894055
                                                                                                                                     |                                                                                                                               
atatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGt                                                                                                                            <  1:485975/139‑1 (MQ=255)
           gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgcg                                                                                                                 <  1:157495/139‑1 (MQ=255)
           gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgcg                                                                                                                 <  1:450586/139‑1 (MQ=255)
                            tGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa                                                                                                >  1:312356‑M1/1‑138 (MQ=255)
                              aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGatt                                                                                              >  2:61062‑M1/1‑136 (MQ=255)
                                    cTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccg                                                                                        >  2:666226‑M1/1‑130 (MQ=255)
                                    cTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa                                                                                                <  1:564426‑M1/131‑2 (MQ=255)
                                    cTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa                                                                                                >  2:564426‑M1/1‑130 (MQ=255)
                                      gTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggc                                                                                      <  2:553231‑M1/139‑12 (MQ=255)
                                       ttCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCCCGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggct                                                                                     >  2:781219‑M1/1‑127 (MQ=255)
                                       ttCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggct                                                                                     >  1:397243‑M1/1‑127 (MQ=255)
                                        tCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctg                                                                                    >  1:245444‑M1/1‑126 (MQ=255)
                                        tCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctg                                                                                    >  2:7547‑M1/1‑126 (MQ=255)
                                                 gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttg                                                                           >  1:92358‑M1/1‑117 (MQ=255)
                                                 gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccg                                                                                        <  1:28587‑M1/126‑10 (MQ=255)
                                                 gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccg                                                                                        >  2:28587‑M1/1‑117 (MQ=255)
                                                   gcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgag                                                                         <  1:993151‑M1/139‑25 (MQ=255)
                                                                 catcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcaga                                                           >  1:579946‑M1/1‑101 (MQ=255)
                                                                     atTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacgg                                                       >  2:782827‑M1/1‑97 (MQ=255)
                                                                              gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac                                              <  1:7547‑M1/139‑52 (MQ=255)
                                                                               tcACGGCCTTTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacg                                             >  2:1040235‑M1/1‑87 (MQ=255)
                                                                                    gCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGa                                                                                                        >  1:873169/1‑75 (MQ=255)
                                                                                    gCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGa                                                                                                        <  2:873169/75‑1 (MQ=255)
                                                                                       tgtgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag                                                            <  1:1012098‑M1/116‑38 (MQ=255)
                                                                                       tgtgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag                                                            >  2:1012098‑M1/1‑79 (MQ=255)
                                                                                         tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc                                   >  2:881434‑M1/1‑77 (MQ=255)
                                                                                         tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa                                                          >  1:578519‑M1/1‑77 (MQ=255)
                                                                                         tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa                                                          <  2:578519‑M1/116‑40 (MQ=255)
                                                                                             aTCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTg                                                                                                                    >  2:530260/1‑54 (MQ=255)
                                                                                             aTCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTg                                                                                                                    <  1:530260/54‑1 (MQ=255)
                                                                                               cTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtct                             >  1:601132‑M1/1‑71 (MQ=255)
                                                                                                     ggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggt                                                      <  1:279903‑M1/108‑44 (MQ=255)
                                                                                                     ggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttcagcgccagacggcagaacggt                                                      >  2:279903‑M1/1‑65 (MQ=255)
                                                                                                      gcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcg                      <  1:782827‑M1/139‑76 (MQ=255)
                                                                                                          tGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctca                  <  1:61062‑M1/139‑80 (MQ=255)
                                                                                                                 aGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgga           <  2:312356‑M1/139‑87 (MQ=255)
                                                                                                                   gAGTCTGAGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggccgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtctgttttcgctcacaccggaag         >  2:727777‑M1/1‑51 (MQ=255)
                                                                                                                    aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggacgg        >  2:545329‑M1/1‑50 (MQ=255)
                                                                                                                    aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct                    <  2:933246‑M1/127‑78 (MQ=255)
                                                                                                                    aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct                    >  1:933246‑M1/1‑50 (MQ=255)
                                                                                                                     gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt       >  2:1096310‑M1/1‑49 (MQ=255)
                                                                                                                     gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt       <  1:1096310‑M1/139‑91 (MQ=255)
                                                                                                                         gCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcactcacaccggaaggtaact   <  1:1040235‑M1/139‑95 (MQ=255)
                                                                                                                          cGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaactt  <  1:1056914‑M1/139‑96 (MQ=255)
                                                                                                                                     |                                                                                                                               
ATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAA  >  NC_000913/2893795‑2894055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: