Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,917	G→A	G112S (GGC→AGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,917	0	G	A	100.0%	114.5 / NA	36	G112S (GGC→AGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

CTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTT  >  NC_000913/2935789‑2936048                                                                                                                                 |                                                                                                                                    ctgctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACg                                                                                                                           >  2:1059011/1‑139 (MQ=255)         gaagaaAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgcg                                                                                                                   <  2:229770/139‑1 (MQ=255)                       cagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                     <  1:1059011/139‑1 (MQ=255)                       cagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                     <  2:166535/139‑1 (MQ=255)                             gAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGct                                                                                               >  1:554379/1‑139 (MQ=255)                                    ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCgg                                                                                                                     >  1:1043092/1‑110 (MQ=255)                                    ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCgg                                                                                                                     <  2:1043092/110‑1 (MQ=255)                                      ccTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCt                                                                                      <  2:78534/139‑1 (MQ=255)                                      ccTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCt                                                                                      >  1:1008449/1‑139 (MQ=255)                                        tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTca                                                                                    <  1:818674/139‑1 (MQ=255)                                         cACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGc                                                                                                >  2:597777/1‑126 (MQ=255)                                         cACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGc                                                                                                <  1:597777/126‑1 (MQ=255)                                                    tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                        <  2:1008449/139‑1 (MQ=255)                                                    tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                        >  1:909459/1‑139 (MQ=255)                                                      aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCa                                                                      >  2:722418/1‑139 (MQ=255)                                                         ccTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATcc                                                                   >  1:666434/1‑139 (MQ=255)                                                              cTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                                              <  2:909459/139‑1 (MQ=255)                                                                ggTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAtt                                                            <  2:811394/139‑1 (MQ=255)                                                                          cAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTAc                                                  <  1:349496/139‑1 (MQ=255)                                                                                          aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                  <  1:610685/139‑1 (MQ=255)                                                                                          aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                  <  2:520367/139‑1 (MQ=255)                                                                                                   aCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATg                         >  1:848516/1‑139 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCggtg                                                                                                                   <  2:667142/44‑3 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGgcg                                                                                                                   >  1:667142/1‑44 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                                              >  2:344608/1‑97 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                                              <  1:344608/97‑1 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca                    >  1:328484/1‑139 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca                    >  2:525502/1‑139 (MQ=255)                                                                                                        cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca                    <  2:666434/139‑1 (MQ=255)                                                                                                         ccgAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                   <  2:506511/139‑1 (MQ=255)                                                                                                                   tttGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGc         >  1:398318/1‑139 (MQ=255)                                                                                                                   tttGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGc         <  2:848516/139‑1 (MQ=255)                                                                                                                     tGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTATCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCg       <  1:751506/139‑1 (MQ=255)                                                                                                                          cTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGtt  >  1:864438/1‑139 (MQ=255)                                                                                                                            ttAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                  >  1:91063/1‑105 (MQ=255)                                                                                                                            ttAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                  <  2:91063/105‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    CTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTT  >  NC_000913/2935789‑2936048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑