Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,856	G→A	*73* (TAG→TAA)	ygeI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,856	0	G	A	100.0%	192.3 / NA	60	*73* (TAG→TAA)	ygeI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/28);  total (32/28)

CAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTT  >  NC_000913/2993730‑2993989                                                                                                                               |                                                                                                                                      cAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaa                                                                                                                           <  1:205182/139‑1 (MQ=255) cAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaa                                                                                                                           >  2:205182/1‑139 (MQ=255) cAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaa                                                                                                                           >  1:640338/1‑139 (MQ=255) cAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaa                                                                                                                           >  1:51871/1‑139 (MQ=255)         gTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCa                                                                                                                   >  1:920871/1‑139 (MQ=255)                 gATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           <  2:62938/139‑1 (MQ=255)                 gATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           <  1:489614/139‑1 (MQ=255)                  aTACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          <  2:181801/139‑1 (MQ=255)                    aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                        <  2:466230/139‑1 (MQ=255)                        aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                               <  1:848988/112‑1 (MQ=255)                        aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                               >  2:848988/1‑112 (MQ=255)                        aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                    >  1:432359/1‑139 (MQ=255)                         tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                   >  1:122111/1‑139 (MQ=255)                            aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                   <  1:542946/136‑1 (MQ=255)                            aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                   >  2:542946/1‑136 (MQ=255)                                cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                            >  2:150005/1‑139 (MQ=255)                                       ttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGt                                                                                     <  2:920871/139‑1 (MQ=255)                                           aGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                 >  1:764348/1‑139 (MQ=255)                                              ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttc                                                                              >  1:458696/1‑139 (MQ=255)                                               tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                               <  2:231419/89‑1 (MQ=255)                                               tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                               >  1:231419/1‑89 (MQ=255)                                               tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           >  2:930851/1‑109 (MQ=255)                                               tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           <  1:930851/109‑1 (MQ=255)                                                 aaCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                         >  2:846007/1‑93 (MQ=255)                                                 aaCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                         <  1:846007/93‑1 (MQ=255)                                                  aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                          <  1:1007551/139‑1 (MQ=255)                                                   cATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                         <  2:764348/139‑1 (MQ=255)                                                   cATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                         <  2:458696/139‑1 (MQ=255)                                                       gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          <  2:973127/102‑1 (MQ=255)                                                       gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          >  1:973127/1‑102 (MQ=255)                                                               cTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACAt                                                             <  2:1042662/139‑1 (MQ=255)                                                               cTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACAt                                                             <  2:201308/139‑1 (MQ=255)                                                                ttttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCt                                                                    >  1:868779/1‑131 (MQ=255)                                                                ttttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCt                                                                    <  2:868779/131‑1 (MQ=255)                                                                 tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTg                                                                                                                                <  2:265080/70‑1 (MQ=255)                                                                 tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTg                                                                                                                                >  1:265080/1‑70 (MQ=255)                                                                 tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAc                                                                                                               <  1:916331/87‑1 (MQ=255)                                                                 tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAc                                                                                                               >  2:916331/1‑87 (MQ=255)                                                                                     agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAat                                                     <  2:32515/125‑1 (MQ=255)                                                                                     agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAat                                                     >  1:32515/1‑125 (MQ=255)                                                                                      gaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTtat                                                                                                  >  2:327321/1‑79 (MQ=255)                                                                                      gaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTtat                                                                                                  <  1:327321/79‑1 (MQ=255)                                                                                       aagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAg                                     >  2:698429/1‑139 (MQ=255)                                                                                          aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATACCGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  >  2:221336/1‑139 (MQ=255)                                                                                          aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  >  1:1029809/1‑139 (MQ=255)                                                                                            aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTaaa                                >  2:954952/1‑139 (MQ=255)                                                                                                     tcACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaa                                       >  2:804595/1‑123 (MQ=255)                                                                                                     tcACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaa                                       <  1:804595/123‑1 (MQ=255)                                                                                                        ccTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATc                    >  1:1113004/1‑139 (MQ=255)                                                                                                         cTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCt                   >  1:525234/1‑139 (MQ=255)                                                                                                           tACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTc                 >  1:348052/1‑139 (MQ=255)                                                                                                           tACTATAAAATTGAGTGGTAAACGATTAACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTc                 <  2:640338/139‑1 (MQ=255)                                                                                                            aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCa                <  2:891848/139‑1 (MQ=255)                                                                                                                taAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtt            <  2:122111/139‑1 (MQ=255)                                                                                                                       gAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtt            >  1:429434/1‑132 (MQ=255)                                                                                                                       gAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtt            <  2:429434/132‑1 (MQ=255)                                                                                                                        aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat    >  2:857943/1‑139 (MQ=255)                                                                                                                          tGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                                   <  1:447210/106‑1 (MQ=255)                                                                                                                          tGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                                   >  2:447210/1‑106 (MQ=255)                                                                                                                          tGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt  >  2:337195/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                      CAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTT  >  NC_000913/2993730‑2993989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑