Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,563	G→A	R354H (CGT→CAT)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,563	0	G	A	100.0%	138.9 / NA	44	R354H (CGT→CAT)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

TATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGG  >  NC_000913/262427‑262686                                                                                                                                         |                                                                                                                            tATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCAtt                                                                                                                           >  1:388592/1‑139 (MQ=255)                         gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  2:1114512/139‑1 (MQ=255)                         gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  2:388592/139‑1 (MQ=255)                                                            tGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTGCg                                                                                                   >  2:709032/1‑104 (MQ=255)                                                            tGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  1:709032/104‑1 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTcc                                                                                                    <  1:185081/102‑1 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTcc                                                                                                    >  2:185081/1‑102 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGc                                                                                                  >  1:855467/1‑104 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGc                                                                                                  <  2:855467/104‑1 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTAc                                                                                           <  1:117062/111‑1 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTAc                                                                                           >  2:117062/1‑111 (MQ=255)                                                             gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                               >  1:521560/1‑139 (MQ=255)                                                                 tCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                           <  2:992152/139‑1 (MQ=255)                                                                  cGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACggcggc                                                          <  1:323238/139‑1 (MQ=255)                                                                  cGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACggcggc                                                          <  2:521560/139‑1 (MQ=255)                                                                                       cacacaACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                     <  2:771478/139‑1 (MQ=255)                                                                                       cacacaACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                     >  1:771478/1‑139 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   >  2:836187/1‑67 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  1:836187/67‑1 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  1:1101094/67‑1 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   >  2:1101094/1‑67 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCg                               <  2:953117/135‑1 (MQ=255)                                                                                                 tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCg                               >  1:953117/1‑135 (MQ=255)                                                                                                   cGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGc                         >  2:580570/1‑139 (MQ=255)                                                                                                      tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACg                                                                                    <  1:502401/77‑1 (MQ=255)                                                                                                      tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACg                                                                                    >  2:502401/1‑77 (MQ=255)                                                                                                      tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGcaca                      >  1:539695/1‑139 (MQ=255)                                                                                                        cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                    <  1:427688/43‑1 (MQ=255)                                                                                                        cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                    >  2:427688/1‑43 (MQ=255)                                                                                                        cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGAGGAAGTGGCGGTAAGcacaca                    >  1:495226/1‑139 (MQ=255)                                                                                                                     cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                     >  1:1086786/1‑109 (MQ=255)                                                                                                                     cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                     <  2:1086786/109‑1 (MQ=255)                                                                                                                     cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAg                          >  2:507042/1‑120 (MQ=255)                                                                                                                     cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAg                          <  1:507042/120‑1 (MQ=255)                                                                                                                     cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg       >  1:661014/1‑139 (MQ=255)                                                                                                                      gATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgc      <  2:539695/139‑1 (MQ=255)                                                                                                                         atGCGCAACGCCCAGCATTTTGTTAACGAAGTGGAATCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTg   >  1:252726/1‑139 (MQ=255)                                                                                                                          tGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCTACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTgg  <  2:495226/139‑1 (MQ=255)                                                                                                                               aaCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   >  2:1112536/1‑37 (MQ=255)                                                                                                                               aaCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                   <  1:1112536/37‑1 (MQ=255)                                                                                                                                  gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCctct                                                                               <  2:407892/54‑1 (MQ=255)                                                                                                                                  gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCctct                                                                               >  1:407892/1‑54 (MQ=255)                                                                                                                                  gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAg                                                       <  2:1032634/78‑1 (MQ=255)                                                                                                                                  gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAg                                                       >  1:1032634/1‑78 (MQ=255)                                                                                                                                         |                                                                                                                            TATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGG  >  NC_000913/262427‑262686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑