Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,011,414 C→T intergenic (+1/‑47) hyuA → / → yqeA D‑stereospecific phenylhydantoinase/putative amino acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,011,4140CT100.0% 138.0 / NA 43intergenic (+1/‑47)hyuA/yqeAD‑stereospecific phenylhydantoinase/putative amino acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/25);  total (18/25)

TACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAA  >  NC_000913/3011284‑3011529
                                                                                                                                  |                                                                                                                   
tACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             <  1:84603/139‑1 (MQ=255)
tACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             >  1:140671/1‑139 (MQ=255)
tACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             <  1:942130/139‑1 (MQ=255)
tACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             <  1:1046068/139‑1 (MQ=255)
 aCCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAg                                                                                                            >  1:321520/1‑139 (MQ=255)
 aCCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGC‑AACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           <  2:1112553/139‑1 (MQ=255)
  ccTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           <  2:321520/139‑1 (MQ=255)
  ccTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           <  2:392202/139‑1 (MQ=255)
     gTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGAt                                                                                                        <  2:95033/139‑1 (MQ=255)
      tCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGAt                                                                                                        >  1:747027/1‑138 (MQ=255)
      tCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGAt                                                                                                        <  2:747027/138‑1 (MQ=255)
      tCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATc                                                                                                       >  1:269033/1‑139 (MQ=255)
          ggCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGGCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCtata                                                                                                   <  1:917610/139‑1 (MQ=255)
           gcgcGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATac                                                                                                  <  2:194859/139‑1 (MQ=255)
            cgcgATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCtata                                                                                                   <  2:155282/137‑1 (MQ=255)
            cgcgATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCtata                                                                                                   >  1:155282/1‑137 (MQ=255)
                aTTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             <  2:130753/123‑1 (MQ=255)
                aTTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCa                                                                                                             >  1:130753/1‑123 (MQ=255)
                             tATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCtata                                                                                                   <  1:674744/120‑1 (MQ=255)
                             tATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCATTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCtata                                                                                                   >  2:674744/1‑120 (MQ=255)
                              aTCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGAtgagt                                                                               <  1:232727/139‑1 (MQ=255)
                              aTCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGAtgagt                                                                               <  1:156748/139‑1 (MQ=255)
                                    tggtgAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGAt                                                                                                        >  1:373513/1‑108 (MQ=255)
                                    tggtgAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGAt                                                                                                        <  2:373513/108‑1 (MQ=255)
                                         aaaCGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATg                                                                    <  2:269033/139‑1 (MQ=255)
                                           aCGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAg                                                                  <  2:140671/139‑1 (MQ=255)
                                                                tttACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTaa                                                               >  1:693444/1‑121 (MQ=255)
                                                                tttACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTaa                                                               <  2:693444/121‑1 (MQ=255)
                                                                         aaaGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGaa                                    >  1:324588/1‑139 (MQ=255)
                                                                         aaaGCCGGGCGAGGTCGTTCCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGaa                                    >  1:263759/1‑139 (MQ=255)
                                                                          aaGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAAtgatga                                                                                 <  2:960639/93‑1 (MQ=255)
                                                                          aaGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAAtgatga                                                                                 >  1:960639/1‑93 (MQ=255)
                                                                           aGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATg                                  >  1:167575/1‑139 (MQ=255)
                                                                           aGCCGAGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAAtgatga                                                                                 >  2:169365/1‑92 (MQ=255)
                                                                           aGCCGAGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAAtgatga                                                                                 <  1:169365/92‑1 (MQ=255)
                                                                            gCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           <  1:999416/65‑1 (MQ=255)
                                                                            gCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           >  2:999416/1‑65 (MQ=255)
                                                                                ggCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCAc                                                                                                                  >  2:185684/1‑54 (MQ=255)
                                                                                ggCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCAc                                                                                                                  <  1:185684/54‑1 (MQ=255)
                                                                                 gCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           <  1:934922/60‑1 (MQ=255)
                                                                                 gCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAgg                                                                                                           >  2:934922/1‑60 (MQ=255)
                                                                                 gCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTgg                            >  2:168333/1‑139 (MQ=255)
                                                                                                           cGTTTGTCCCTCCCGTGCTCTAATCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGaaa  <  2:456292/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                   
TACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAA  >  NC_000913/3011284‑3011529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑