Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,032	G→A	S222S (TCG→TCA)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,032	0	G	A	100.0%	159.2 / NA	49	S222S (TCG→TCA)	uacT	uric acid permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

ATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTG  >  NC_000913/3031900‑3032167                                                                                                                                     |                                                                                                                                        atatGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGgatg                                                                                                                                   <  1:304878/139‑1 (MQ=255)       tAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGatgaat                                                                                                                             <  1:73313/139‑1 (MQ=255)            ccGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGt                                                                                                                        >  1:980024/1‑139 (MQ=255)              gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCa                                                                                                                      >  2:599498/1‑139 (MQ=255)               tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCaa                                                                                                                     >  1:1104870/1‑139 (MQ=255)                ttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAAt                                                                                                                    >  1:775569/1‑139 (MQ=255)                    ttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTAGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                                <  1:714111/139‑1 (MQ=255)                     taGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATc                                                                                                               <  2:693606/139‑1 (MQ=255)                         tATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                           >  2:135222/1‑139 (MQ=255)                           tctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATg                                                                                                                                       >  1:531563/1‑109 (MQ=255)                              ccTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa                                                                                                      <  2:863995/139‑1 (MQ=255)                                       tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGAtgaatgaa                                                                                                                          <  1:1023573/110‑1 (MQ=255)                                       tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGAtgaatgaa                                                                                                                          >  2:1023573/1‑110 (MQ=255)                                         ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCAt                                                                                           >  2:210516/1‑139 (MQ=255)                                         ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCAt                                                                                           >  2:366161/1‑139 (MQ=255)                                                ttATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGc                                                                                    <  1:599498/139‑1 (MQ=255)                                                 tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCg                                                                                   <  1:230413/139‑1 (MQ=255)                                                 tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCg                                                                                   <  1:210516/139‑1 (MQ=255)                                                   tCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                    >  1:893033/1‑120 (MQ=255)                                                   tCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                    <  2:893033/120‑1 (MQ=255)                                                        cTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTa                                                                            >  1:1026020/1‑139 (MQ=255)                                                           aTTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGc                                                                         <  2:51521/139‑1 (MQ=255)                                                                  gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTc                                                                  >  2:3200/1‑139 (MQ=255)                                                                  gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTc                                                                  >  2:127510/1‑139 (MQ=255)                                                                      tGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                           <  1:558496/94‑1 (MQ=255)                                                                      tGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                           >  2:558496/1‑94 (MQ=255)                                                                       gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                                  <  2:220594/118‑1 (MQ=255)                                                                       gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                                  >  1:220594/1‑118 (MQ=255)                                                                              gATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGcc                                                      >  2:880320/1‑139 (MQ=255)                                                                               aTTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCg                                                     <  1:841732/139‑1 (MQ=255)                                                                                         aaCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                           >  1:379598/1‑139 (MQ=255)                                                                                          aCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                          <  1:135222/139‑1 (MQ=255)                                                                                          aCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                          <  1:127510/139‑1 (MQ=255)                                                                                                ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTc                                    >  1:259150/1‑139 (MQ=255)                                                                                                           gggATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGTGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCa                         >  1:40416/1‑139 (MQ=255)                                                                                                             gATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATg                       >  2:1055371/1‑139 (MQ=255)                                                                                                              aTTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                      >  1:1017306/1‑139 (MQ=255)                                                                                                                tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTg                             <  2:210096/130‑1 (MQ=255)                                                                                                                tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTg                             >  1:210096/1‑130 (MQ=255)                                                                                                                 gTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                   <  2:379598/139‑1 (MQ=255)                                                                                                                       ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                          >  2:239502/1‑110 (MQ=255)                                                                                                                       ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                          <  1:239502/110‑1 (MQ=255)                                                                                                                       ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                   <  1:182084/133‑1 (MQ=255)                                                                                                                       ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                   >  2:182084/1‑133 (MQ=255)                                                                                                                         cTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTa           <  1:1055371/139‑1 (MQ=255)                                                                                                                            tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                                <  2:534315/115‑1 (MQ=255)                                                                                                                            tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                                >  1:534315/1‑115 (MQ=255)                                                                                                                            tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatc        >  2:235837/1‑139 (MQ=255)                                                                                                                                  ttCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCgtg  >  2:159362/1‑139 (MQ=255)                                                                                                                                  ttCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCgtg  <  1:686250/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTG  >  NC_000913/3031900‑3032167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑