Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,059,293	T→C	G11G (GGA→GGG)	rpiA ←	ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,059,293	0	T	C	100.0%	184.8 / NA	52	G11G (GGA→GGG)	rpiA	ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATA  >  NC_000913/3059167‑3059420                                                                                                                               |                                                                                                                                tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                     >  1:45929/1‑139 (MQ=255)       ggAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAAt                                                                                                               >  1:722816/1‑139 (MQ=255)                                ttCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                      <  1:847687/139‑1 (MQ=255)                                   aTTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                   <  2:722816/139‑1 (MQ=255)                                            aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                          >  2:208222/1‑139 (MQ=255)                                                 gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                     >  1:522247/1‑139 (MQ=255)                                                 gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                     >  1:423851/1‑139 (MQ=255)                                                 gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                     >  2:422771/1‑139 (MQ=255)                                                  tCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTca                                                                    <  1:422771/139‑1 (MQ=255)                                                   cAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                   <  1:208222/139‑1 (MQ=255)                                                    aaTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCTCCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  <  2:843871/139‑1 (MQ=255)                                                    aaTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  <  2:423851/139‑1 (MQ=255)                                                           tgtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                    <  2:81998/82‑1 (MQ=255)                                                           tgtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                    >  1:81998/1‑82 (MQ=255)                                                           tgtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATta                                                           >  1:219004/1‑139 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                   >  2:545812/1‑113 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                   <  1:545812/113‑1 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                         >  1:879580/1‑139 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                         >  1:781742/1‑139 (MQ=255)                                                                    gTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 >  2:921189/1‑124 (MQ=255)                                                                    gTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 <  1:921189/124‑1 (MQ=255)                                                                        aaCCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAg                                              <  2:879580/139‑1 (MQ=255)                                                                              tACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATGCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTc                                        >  1:524884/1‑139 (MQ=255)                                                                                    cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAccc                                  <  2:45929/139‑1 (MQ=255)                                                                                     acCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  <  1:226506/106‑1 (MQ=255)                                                                                     acCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  >  2:226506/1‑106 (MQ=255)                                                                                         acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTg                             >  1:965473/1‑139 (MQ=255)                                                                                           aaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGc                           >  1:203597/1‑139 (MQ=255)                                                                                              ggTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  >  1:290871/1‑97 (MQ=255)                                                                                              ggTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  <  2:290871/97‑1 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGc                       >  1:1098856/1‑139 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGc                       >  1:592860/1‑139 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGc                       >  2:1000828/1‑139 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGc                       >  1:722404/1‑139 (MQ=255)                                                                                                tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGcc                      <  1:33144/139‑1 (MQ=255)                                                                                                  ccGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                    <  2:219004/139‑1 (MQ=255)                                                                                                     ggCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                     >  1:697201/1‑39 (MQ=255)                                                                                                     ggCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                     <  2:697201/39‑1 (MQ=255)                                                                                                          aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTca                                                                    >  2:871222/1‑83 (MQ=255)                                                                                                          aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTca                                                                    <  1:871222/83‑1 (MQ=255)                                                                                                            cATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                   <  1:264280/66‑1 (MQ=255)                                                                                                            cATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                   >  2:264280/1‑66 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 >  2:1113452/1‑81 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 <  1:1113452/81‑1 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt       >  2:578067/1‑139 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt       <  2:781742/139‑1 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt       <  2:203597/139‑1 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  <  1:357292/75‑1 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                  >  2:357292/1‑75 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 >  2:756172/1‑76 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                 <  1:756172/76‑1 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATa  >  1:442225/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATA  >  NC_000913/3059167‑3059420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑