Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,079,798 G→A A613V (GCG→GTG)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,079,7980GA100.0% 144.4 / NA 45A613V (GCG→GTG) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/20);  total (25/20)

CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC  >  NC_000913/3079665‑3079931
                                                                                                                                     |                                                                                                                                     
cGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCt                                                                                                                                  >  1:471987/1‑139 (MQ=255)
           tATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg                                                                                                                       >  2:913000/1‑139 (MQ=255)
                  aGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTa                                                                                                                >  1:1009063/1‑139 (MQ=255)
                      aaGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATAGTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTGACCg                                                                                                            >  1:734296/1‑139 (MQ=255)
                       aGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGc                                                                                                           >  2:353627/1‑139 (MQ=255)
                             aCTCTTCAAACTGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCgg                                                                                                     <  1:722982/139‑1 (MQ=255)
                                             ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg                                                                                                                     <  1:157314/107‑1 (MQ=255)
                                             ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg                                                                                                                     >  2:157314/1‑107 (MQ=255)
                                              tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg                                                                                                                       <  1:1063716/104‑1 (MQ=255)
                                              tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg                                                                                                                       >  2:1063716/1‑104 (MQ=255)
                                              tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGt                                                                                    <  1:913000/139‑1 (MQ=255)
                                                    ggAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagcag                                                                              <  1:1054596/139‑1 (MQ=255)
                                                                      gtggtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATg                                                            <  1:286264/139‑1 (MQ=255)
                                                                               ccGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAg                                                   <  1:7061/139‑1 (MQ=255)
                                                                                   cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg                                                                                                                       <  2:54125/67‑1 (MQ=37)
                                                                                   cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg                                                                                                                       >  1:54125/1‑67 (MQ=255)
                                                                                    gaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGc                                              >  2:75540/1‑139 (MQ=255)
                                                                                         cACCGTTCAGGCCTACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGTAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa                                         <  2:734296/139‑1 (MQ=255)
                                                                                              ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                    >  1:1037240/1‑139 (MQ=255)
                                                                                                        cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg                                                                                                                     >  1:418613/1‑48 (MQ=255)
                                                                                                        cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg                                                                                                                     <  2:418613/48‑1 (MQ=255)
                                                                                                        cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                          >  1:852827/1‑139 (MQ=255)
                                                                                                                  ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc                                                 >  2:407367/1‑106 (MQ=255)
                                                                                                                  ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc                                                 <  1:407367/106‑1 (MQ=255)
                                                                                                                     gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                               >  2:292418/1‑121 (MQ=255)
                                                                                                                     gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                               <  1:292418/121‑1 (MQ=255)
                                                                                                                     gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca             >  2:495966/1‑139 (MQ=255)
                                                                                                                     gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca             >  1:1036933/1‑139 (MQ=255)
                                                                                                                      tagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcag            <  2:724001/139‑1 (MQ=255)
                                                                                                                       agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg                                                                                              >  1:844694/1‑56 (MQ=255)
                                                                                                                       agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg                                                                                              <  2:844694/56‑1 (MQ=255)
                                                                                                                       agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc                                                 <  2:99306/101‑1 (MQ=255)
                                                                                                                       agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc                                                 >  1:99306/1‑101 (MQ=255)
                                                                                                                       agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt           <  2:1036933/139‑1 (MQ=255)
                                                                                                                        gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa                                                  <  2:258526/99‑1 (MQ=255)
                                                                                                                        gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa                                                  >  1:258526/1‑99 (MQ=255)
                                                                                                                          cAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag        >  2:398251/1‑139 (MQ=255)
                                                                                                                           aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt       >  2:600427/1‑139 (MQ=255)
                                                                                                                           aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt       >  2:698706/1‑139 (MQ=255)
                                                                                                                           aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt       <  2:1009063/139‑1 (MQ=255)
                                                                                                                                tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt           >  1:793480/1‑130 (MQ=255)
                                                                                                                                tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt           <  2:793480/130‑1 (MQ=255)
                                                                                                                                tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc  >  1:240595/1‑139 (MQ=255)
                                                                                                                                  cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg                                                                                                  >  2:434443/1‑41 (MQ=255)
                                                                                                                                  cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg                                                                                                  <  1:434443/41‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC  >  NC_000913/3079665‑3079931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: