Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,079,798 | G→A | A613V (GCG→GTG) | tktA ← | transketolase 1, thiamine triphosphate‑binding |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,079,798 | 0 | G | A | 100.0% | 144.4 / NA | 45 | A613V (GCG→GTG) | tktA | transketolase 1, thiamine triphosphate‑binding |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/20); total (25/20) |
CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC > NC_000913/3079665‑3079931 | cGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCt > 1:471987/1‑139 (MQ=255) tATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg > 2:913000/1‑139 (MQ=255) aGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTa > 1:1009063/1‑139 (MQ=255) aaGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATAGTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTGACCg > 1:734296/1‑139 (MQ=255) aGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGc > 2:353627/1‑139 (MQ=255) aCTCTTCAAACTGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCgg < 1:722982/139‑1 (MQ=255) ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg < 1:157314/107‑1 (MQ=255) ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg > 2:157314/1‑107 (MQ=255) tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg < 1:1063716/104‑1 (MQ=255) tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg > 2:1063716/1‑104 (MQ=255) tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGt < 1:913000/139‑1 (MQ=255) ggAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagcag < 1:1054596/139‑1 (MQ=255) gtggtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATg < 1:286264/139‑1 (MQ=255) ccGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAg < 1:7061/139‑1 (MQ=255) cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg < 2:54125/67‑1 (MQ=37) cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg > 1:54125/1‑67 (MQ=255) gaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGc > 2:75540/1‑139 (MQ=255) cACCGTTCAGGCCTACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGTAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa < 2:734296/139‑1 (MQ=255) ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca > 1:1037240/1‑139 (MQ=255) cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg > 1:418613/1‑48 (MQ=255) cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg < 2:418613/48‑1 (MQ=255) cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt > 1:852827/1‑139 (MQ=255) ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc > 2:407367/1‑106 (MQ=255) ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc < 1:407367/106‑1 (MQ=255) gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc > 2:292418/1‑121 (MQ=255) gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc < 1:292418/121‑1 (MQ=255) gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca > 2:495966/1‑139 (MQ=255) gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca > 1:1036933/1‑139 (MQ=255) tagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcag < 2:724001/139‑1 (MQ=255) agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:844694/1‑56 (MQ=255) agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg < 2:844694/56‑1 (MQ=255) agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc < 2:99306/101‑1 (MQ=255) agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc > 1:99306/1‑101 (MQ=255) agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt < 2:1036933/139‑1 (MQ=255) gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa < 2:258526/99‑1 (MQ=255) gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa > 1:258526/1‑99 (MQ=255) cAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag > 2:398251/1‑139 (MQ=255) aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt > 2:600427/1‑139 (MQ=255) aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt > 2:698706/1‑139 (MQ=255) aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt < 2:1009063/139‑1 (MQ=255) tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt > 1:793480/1‑130 (MQ=255) tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt < 2:793480/130‑1 (MQ=255) tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc > 1:240595/1‑139 (MQ=255) cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg > 2:434443/1‑41 (MQ=255) cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg < 1:434443/41‑1 (MQ=255) | CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC > NC_000913/3079665‑3079931 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |