Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,031	T→C	Q627Q (CAA→CAG)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,031	0	T	C	100.0%	150.9 / NA	42	Q627Q (CAA→CAG)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGC  >  NC_000913/3083903‑3084162                                                                                                                                 |                                                                                                                                    gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           <  2:529221/139‑1 (MQ=255)   tcaaTTAATCGGAATCAAGTAACGTAGCAGCTTCTCATCTTCAATATAGGAATACTTGTACAAACCTGCCTCGAACTCTTGAAGGAACTGTTGTGGCAGTCCAGCATCAATTTCGGTTTTCTTCACCTGATCGCGGAAc                                                                                                                         <  2:486409/137‑1 (MQ=255)          aGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTa                                                                                                                  >  1:905306/1‑139 (MQ=255)               aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTACAACCGTACAAACCTGCCTCGAACTCTTCAAGGGACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           >  1:118778/1‑125 (MQ=255)               aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTACAACCGTACAAACCTGCCTCGAACTCTTCAAGGGACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           <  2:118778/125‑1 (MQ=255)                          acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATc                                                                                                  <  2:788926/139‑1 (MQ=255)                             ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:331693/139‑1 (MQ=255)                             ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  2:450098/1‑139 (MQ=255)                             ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  2:615531/1‑139 (MQ=255)                                      cATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  2:137738/132‑1 (MQ=255)                                      cATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  1:137738/1‑132 (MQ=255)                                                         aCCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc                                                                                         <  2:969143/117‑1 (MQ=255)                                                         aCCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc                                                                                         >  1:969143/1‑117 (MQ=255)                                                          ccGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACgg                                                                  >  2:652477/1‑139 (MQ=255)                                                             tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:500744/107‑1 (MQ=255)                                                             tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  1:500744/1‑107 (MQ=255)                                                             tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGcc                                                                      >  1:300176/1‑132 (MQ=255)                                                             tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGcc                                                                      <  2:300176/132‑1 (MQ=255)                                                             tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTAt                                                               >  2:1011715/1‑139 (MQ=255)                                                              aCAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:357488/106‑1 (MQ=255)                                                              aCAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  1:357488/1‑106 (MQ=255)                                                               cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAGGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  2:538689/1‑107 (MQ=255)                                                               cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAGGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  1:538689/107‑1 (MQ=255)                                                               cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCg                                                             >  2:1084279/1‑139 (MQ=255)                                                               cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCg                                                             >  1:625068/1‑139 (MQ=255)                                                                    cTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  1:893455/1‑102 (MQ=255)                                                                    cTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  2:893455/102‑1 (MQ=255)                                                                          cGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGa                                                  <  2:905306/139‑1 (MQ=255)                                                                          cGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGa                                                  <  1:652477/139‑1 (MQ=255)                                                                                 ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           >  2:974431/1‑139 (MQ=255)                                                                                 ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           <  1:149138/139‑1 (MQ=255)                                                                                 ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           >  1:864316/1‑139 (MQ=255)                                                                                       gAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttctacttct                                     <  1:450098/139‑1 (MQ=255)                                                                                         aCTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGAc                                                 <  2:862865/125‑1 (MQ=255)                                                                                         aCTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGAc                                                 >  1:862865/1‑125 (MQ=255)                                                                                           tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  2:582045/1‑139 (MQ=255)                                                                                           tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  1:730581/1‑139 (MQ=255)                                                                                           tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  1:1029472/1‑139 (MQ=255)                                                                                            gttgttGCAGTACAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCt                                <  1:720609/139‑1 (MQ=255)                                                                                                            aTCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACg                <  1:582045/139‑1 (MQ=255)                                                                                                                     ggTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCa       >  2:863004/1‑139 (MQ=255)                                                                                                                          tcttcACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGc  <  2:1029472/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGC  >  NC_000913/3083903‑3084162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑