Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,823 G→A *231* (TAG→TAA yghT → putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8230GA100.0% 96.3 / NA 31*231* (TAG→TAAyghTputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/14);  total (17/14)

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134686‑3134946
                                                                                                                                         |                                                                                                                           
aaGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                           <  2:1079104/139‑1 (MQ=255)
    ttGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCaaaa                                                                                                                       <  2:419962/139‑1 (MQ=255)
             cGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATTACCGCGCGGGTTATCACTAATCAAAAATGGAAATg                                                                                                              >  2:275068/1‑139 (MQ=255)
                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:275068/139‑1 (MQ=255)
                  caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGa                                                                                                         >  1:904072/1‑139 (MQ=255)
                          cTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAgg                                                                                                 >  1:587159/1‑139 (MQ=255)
                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                              >  2:346098/1‑139 (MQ=255)
                               gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            <  2:587159/139‑1 (MQ=255)
                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAGGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:619159/114‑1 (MQ=255)
                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:619159/1‑114 (MQ=255)
                                                     cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                      <  1:346098/139‑1 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                       >  1:1022578/1‑120 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                       <  2:1022578/120‑1 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                 >  1:222329/1‑126 (MQ=255)
                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                 <  2:222329/126‑1 (MQ=255)
                                                        tGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                           <  2:929025/83‑1 (MQ=255)
                                                        tGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                           >  1:929025/1‑83 (MQ=255)
                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                            <  2:904072/139‑1 (MQ=255)
                                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                               >  1:1114345/1‑139 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                 <  1:366529/117‑1 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                 >  2:366529/1‑117 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                            <  1:424451/122‑1 (MQ=255)
                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                            >  2:424451/1‑122 (MQ=255)
                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGCTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGCGCATTTTCAGGAAGGTAGAATCAACTGCAATGCTATCCAGTACTGCCAACCagcaag                                   >  1:229017/1‑139 (MQ=255)
                                                                                              aaaaaGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAt                             >  1:215172/1‑139 (MQ=255)
                                                                                                                       cgcgcgGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc    >  1:59170/1‑139 (MQ=255)
                                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                 >  2:1089094/5‑60 (MQ=255)
                                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                 <  1:1089094/56‑1 (MQ=255)
                                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                 <  1:549533/72‑1 (MQ=255)
                                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                 >  2:549533/5‑76 (MQ=255)
                                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc  >  1:1029180/5‑139 (MQ=255)
                                                                                                                                         |                                                                                                                           
AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134686‑3134946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: