Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,675	G→A	G112G (GGC→GGT)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,675	0	G	A	100.0%	208.2 / NA	64	G112G (GGC→GGT)	yhaO	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/33);  total (31/33)

TCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAA  >  NC_000913/3257550‑3257809                                                                                                                              |                                                                                                                                       tCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTAc                                                                                                                           >  1:622417/1‑139 (MQ=255)  cTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTAcc                                                                                                                          <  2:379389/139‑1 (MQ=255)         cAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATaa                                                                                                                   >  1:964400/1‑139 (MQ=255)         cAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATaa                                                                                                                   >  2:697423/1‑139 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                     <  1:75256/135‑1 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                     >  2:75256/1‑135 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATa                                                                                                                    >  2:678387/1‑136 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                                 >  1:1052094/1‑139 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                                 >  1:193321/1‑139 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                                 >  2:822913/1‑139 (MQ=255)           aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACACGATACCCCAGTTTTTACCTAAATa                                                                                                                    <  1:678387/136‑1 (MQ=255)                    tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATa                                                                                                                    <  1:861714/127‑1 (MQ=255)                    tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATa                                                                                                                    >  2:861714/1‑127 (MQ=255)                           gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCggg                                                                                                 <  1:1031546/139‑1 (MQ=255)                            aaGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  1:822913/139‑1 (MQ=255)                            aaGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  2:964400/139‑1 (MQ=255)                             aGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTa                                                                                               <  1:136325/139‑1 (MQ=255)                                 aTGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                           <  2:1052094/139‑1 (MQ=255)                                        tAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       >  1:353524/1‑120 (MQ=255)                                        tAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       <  2:353524/120‑1 (MQ=255)                                         aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                          >  1:718553/1‑132 (MQ=255)                                         aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                          <  2:718553/132‑1 (MQ=255)                                                 cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAt                                                                                     >  1:1062242/1‑129 (MQ=255)                                                 cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAt                                                                                     <  2:1062242/129‑1 (MQ=255)                                                 cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                           >  2:145209/1‑139 (MQ=255)                                                          gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  1:145209/139‑1 (MQ=255)                                                          gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  2:464354/139‑1 (MQ=255)                                                              tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTAtt                                                              >  2:473979/1‑139 (MQ=255)                                                               ggCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATta                                                             >  2:954799/1‑139 (MQ=255)                                                                      gAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACa                                                      <  1:339704/139‑1 (MQ=255)                                                                          aGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCg                                                  <  1:1057673/139‑1 (MQ=255)                                                                              gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAGCCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                       <  1:240709/114‑1 (MQ=255)                                                                              gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAGCCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                       >  2:240709/1‑114 (MQ=255)                                                                              gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACAGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGa                                              <  2:22713/139‑1 (MQ=255)                                                                                  aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                >  2:585205/1‑85 (MQ=255)                                                                                  aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  1:585205/85‑1 (MQ=255)                                                                                               gCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 >  2:1098434/1‑87 (MQ=255)                                                                                               gCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 <  1:1098434/87‑1 (MQ=255)                                                                                               gCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                       <  2:391379/97‑1 (MQ=255)                                                                                               gCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                       >  1:391379/1‑97 (MQ=255)                                                                                                  ttACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  1:320906/99‑1 (MQ=255)                                                                                                  ttACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  >  2:320906/1‑99 (MQ=255)                                                                                                     cGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       >  1:1051887/1‑59 (MQ=255)                                                                                                     cGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       <  2:1051887/59‑1 (MQ=255)                                                                                                     cGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGa                                                                             >  2:707804/1‑85 (MQ=255)                                                                                                     cGAAATAGAGCGCACCTAACAAGATACCCCAGTTTTTCCCTAAATAACTGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGa                                                                             <  1:707804/85‑1 (MQ=255)                                                                                                          tAGAGCGCACCTAATAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       >  1:675962/1‑54 (MQ=255)                                                                                                          tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                       <  2:675962/54‑1 (MQ=255)                                                                                                          tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                                                                         <  2:106245/68‑1 (MQ=255)                                                                                                          tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                                                                         >  1:106245/1‑68 (MQ=255)                                                                                                              gcgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGa              >  2:508493/1‑139 (MQ=255)                                                                                                               cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  2:309977/56‑1 (MQ=255)                                                                                                               cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  1:856739/56‑1 (MQ=255)                                                                                                               cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                >  1:309977/1‑56 (MQ=255)                                                                                                               cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                >  2:856739/1‑56 (MQ=255)                                                                                                                 cACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGt                           <  1:934849/123‑1 (MQ=255)                                                                                                                 cACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGt                           >  2:934849/1‑123 (MQ=255)                                                                                                                  aCCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                       >  2:1084268/1‑126 (MQ=255)                                                                                                                  aCCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                       <  1:1084268/126‑1 (MQ=255)                                                                                                                    cTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAg                                                                    <  2:811995/79‑1 (MQ=255)                                                                                                                    cTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAg                                                                    >  1:811995/1‑79 (MQ=255)                                                                                                                      aaCAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCa      >  1:508998/1‑139 (MQ=255)                                                                                                                         aGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaa   <  2:97739/139‑1 (MQ=255)                                                                                                                          ggATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaaa  <  1:697423/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                       TCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAA  >  NC_000913/3257550‑3257809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑