Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,602	G→A	*396* (TAG→TAA)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,602	0	G	A	100.0%	166.4 / NA	52	*396* (TAG→TAA)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/31);  total (21/31)

TCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTA  >  NC_000913/3269466‑3269734                                                                                                                                         |                                                                                                                                     tCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAAc                                                                                                                                    <  2:298290/139‑1 (MQ=255)  cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACt                                                                                                                                   >  1:1089304/1‑139 (MQ=255)     gaAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTtaa                                                                                                                                <  1:71731/139‑1 (MQ=255)      aaaGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTtaat                                                                                                                               <  2:122613/139‑1 (MQ=255)      aaaGACGAAAACTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTtaat                                                                                                                               <  1:720443/139‑1 (MQ=255)             aaaaTTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAActtct                                                                                                                        <  1:346437/139‑1 (MQ=255)              aaaTTCTCCGGACAAGGGATTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGATGATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAActtctt                                                                                                                       <  1:193741/139‑1 (MQ=255)                 ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:34839/139‑1 (MQ=255)                 ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:1089304/139‑1 (MQ=255)                   ctcCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaaa                                                                                                                  >  2:754224/1‑139 (MQ=255)                     ccGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt                                                                                                                <  1:542655/139‑1 (MQ=255)                              ggCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTTCCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:758483/126‑1 (MQ=255)                              ggCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    >  1:758483/1‑126 (MQ=255)                                cTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAAt                                                                                                     >  1:288957/1‑139 (MQ=255)                                 tttGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATa                                                                                                    <  1:190380/139‑1 (MQ=255)                                 tttGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATa                                                                                                    <  2:288957/139‑1 (MQ=255)                                      ggTCGGGGAATATAACACATATGAAGCATATAACTCAGAACAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAATTTAATAACTTCTTACAAAGTCTATTATTAATACCGTa                                                                                               <  1:743518/139‑1 (MQ=255)                                               atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                      <  1:57839/139‑1 (MQ=255)                                               atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                      <  2:533961/139‑1 (MQ=255)                                               atatAACACATAAGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                      <  1:1085223/139‑1 (MQ=255)                                                             aaGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTaa                                                                                                      >  2:711294/1‑109 (MQ=255)                                                             aaGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTaa                                                                                                      <  1:711294/109‑1 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAAc                                                                                                                            <  1:276064/82‑1 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAAc                                                                                                                            >  2:276064/1‑82 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaaa                                                                                                                  >  1:338675/1‑92 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaaa                                                                                                                  <  2:338675/92‑1 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                              <  2:681079/96‑1 (MQ=255)                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                              >  1:681079/1‑96 (MQ=255)                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:993343/88‑1 (MQ=255)                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    >  1:993343/1‑88 (MQ=255)                                                                          agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                      >  1:544657/1‑112 (MQ=255)                                                                          agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                      <  2:544657/112‑1 (MQ=255)                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    >  1:210906/1‑80 (MQ=255)                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:210906/80‑1 (MQ=255)                                                                               aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                              >  1:242678/1‑83 (MQ=255)                                                                               aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                              <  2:242678/83‑1 (MQ=255)                                                                                        acaccataaaataCTATACCGATAATTACTACCTATTAAAATCAATATAAACTTAATAACTTCTTACAAAATCTATTATTAATACCCTATATATTTATTCATCTTACCCTATACACCAAAAGCAAAAACTCACTGAcgc                                             <  2:1072130/127‑1 (MQ=255)                                                                                         tACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgcg                                            >  1:158882/1‑139 (MQ=255)                                                                                              gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGttt                                       >  1:250355/1‑139 (MQ=255)                                                                                               agagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTg                                      <  1:754224/139‑1 (MQ=255)                                                                                                 agtattataCGGATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTC‑‑‑TATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                 >  2:645930/6‑139 (MQ=255)                                                                                                        taCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    <  2:207558/52‑1 (MQ=255)                                                                                                        taCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                    >  1:207558/1‑52 (MQ=255)                                                                                                                aTTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGc                     >  2:116281/1‑139 (MQ=255)                                                                                                                  tACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtattta                                                                                       <  2:669951/71‑1 (MQ=255)                                                                                                                  tACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtattta                                                                                       >  1:669951/1‑71 (MQ=255)                                                                                                                    cGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATc                                                                                 >  1:954298/1‑75 (MQ=255)                                                                                                                    cGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATc                                                                                 <  2:954298/75‑1 (MQ=255)                                                                                                                    cGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaaa                 >  1:117920/1‑139 (MQ=255)                                                                                                                    cGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaaa                 <  1:116281/139‑1 (MQ=255)                                                                                                                           ttAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg          <  2:117920/139‑1 (MQ=255)                                                                                                                                tGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATgcg     <  2:250355/139‑1 (MQ=255)                                                                                                                                   aaTATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTa  >  1:432043/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     TCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTA  >  NC_000913/3269466‑3269734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑