Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,296,984 G→A *192* (TAG→TAA yraP → outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,296,9840GA100.0% 86.7 / NA 28*192* (TAG→TAAyraPouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACA  >  NC_000913/3296848‑3297108
                                                                                                                                        |                                                                                                                            
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            <  1:126497/139‑1 (MQ=255)
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                            <  2:663594/139‑1 (MQ=255)
     gTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcccc                                                                                                                       <  1:100333/139‑1 (MQ=255)
             cGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATg                                                                                                               >  1:50170/1‑139 (MQ=255)
               gaaaCGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGcc                                                                                                             <  2:5196/138‑1 (MQ=255)
               aaaaCGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGcc                                                                                                             >  1:263835/1‑139 (MQ=255)
                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc                                                                                                                        >  1:413089/1‑104 (MQ=255)
                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGccc                                                                                                                        <  2:413089/104‑1 (MQ=255)
                                         cTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                   >  2:77671/1‑139 (MQ=255)
                                           ggTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                 <  2:50170/139‑1 (MQ=255)
                                            gTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGCTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                >  1:10742/1‑139 (MQ=255)
                                                cTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                            <  1:573822/139‑1 (MQ=255)
                                                cTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                            <  2:263835/139‑1 (MQ=255)
                                                  gAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                          <  2:10742/139‑1 (MQ=255)
                                                          aGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACa                                                                  <  1:77671/139‑1 (MQ=255)
                                                           gCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACaa                                                                 >  2:456920/1‑139 (MQ=255)
                                                               aaGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             <  1:456920/139‑1 (MQ=255)
                                                               aaGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCg                                                             <  1:181449/139‑1 (MQ=255)
                                                                aGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGt                                                            <  2:67576/139‑1 (MQ=255)
                                                                                                      aGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGttt                      >  2:502852/1‑139 (MQ=255)
                                                                                                          ggTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTccc                  >  1:334273/1‑139 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTCAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACa                                                                  <  2:758709/90‑1 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACa                                                                  >  1:758709/1‑90 (MQ=255)
                                                                                                              aCTACGGCGTTTACATTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCCTATCAGGCCTACAACGATACAGACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCaa              <  1:681409/139‑1 (MQ=255)
                                                                                                                tACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAAt            <  1:502852/139‑1 (MQ=255)
                                                                                                                    gCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                   >  1:154537/1‑64 (MQ=255)
                                                                                                                    gCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                   <  2:154537/64‑1 (MQ=255)
                                                                                                                       tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGAc                            >  2:65087/1‑116 (MQ=255)
                                                                                                                       tttacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGAc                            <  1:65087/116‑1 (MQ=255)
                                                                                                                          acgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAacaaca  >  1:797865/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                            
TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACA  >  NC_000913/3296848‑3297108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: