| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 309,358 | C→T | *223* (TAG→TAA) | ecpB ← | ECP production pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 309,358 | 0 | C | T | 100.0% | 128.7 / NA | 40 | *223* (TAG→TAA) | ecpB | ECP production pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/19); total (21/19) | |||||||||||
GCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGC > NC_000913/309230‑309492 | gcgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACgg > 2:9255/1‑139 (MQ=255) cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACggg > 1:281853/1‑139 (MQ=255) agcagcGCTGGCATCGGGCTGAACGAAC‑AAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACg > 2:940174/1‑124 (MQ=255) agcagcGCTGGCATCGGGCTGAACGAAC‑AAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACg < 1:940174/124‑1 (MQ=255) gcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg < 2:413367/130‑1 (MQ=255) gcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg > 1:413367/1‑130 (MQ=255) cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAAt > 1:240851/1‑125 (MQ=255) cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAAt < 2:240851/125‑1 (MQ=255) ggCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCa < 1:701294/139‑1 (MQ=255) aacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCt < 1:685518/139‑1 (MQ=255) ccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg < 1:9255/139‑1 (MQ=255) cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAac > 1:629011/1‑139 (MQ=255) ttCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAacga < 1:1095263/139‑1 (MQ=255) ccTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGAc < 1:583470/139‑1 (MQ=255) ggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc < 2:629011/139‑1 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAacga > 2:475098/1‑124 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAacga < 1:475098/124‑1 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc > 1:571982/1‑139 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc > 2:586599/1‑139 (MQ=255) tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg > 1:325920/1‑53 (MQ=255) tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg < 2:325920/53‑1 (MQ=255) tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAg > 1:1023304/1‑139 (MQ=255) gtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa < 1:577193/96‑1 (MQ=255) gtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa > 2:577193/1‑96 (MQ=255) aTTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCGGgt > 1:728578/1‑131 (MQ=255) aTTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgt < 2:728578/131‑1 (MQ=255) ttGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTCTCACCCTGCCAAACTGGAACCACTCCTTTGTTATCAGAAGTATCCACGCGGCTAAAACGACGCGACTTGCCCGGCATCAGAAAGTAATtct > 2:1030491/1‑139 (MQ=255) ttGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct > 1:692020/1‑139 (MQ=255) tGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc < 1:870734/118‑1 (MQ=255) tGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc > 2:870734/1‑118 (MQ=255) tGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg < 1:546746/128‑1 (MQ=255) tGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg > 2:546746/1‑128 (MQ=255) gTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTa < 2:281853/139‑1 (MQ=255) ttATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac < 2:571982/139‑1 (MQ=255) tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGcc < 1:575475/45‑1 (MQ=255) tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGcc > 2:575475/1‑45 (MQ=255) cacaGCTATTTATTTCACGGG‑ATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGt > 1:549466/1‑139 (MQ=255) aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTc < 2:94453/72‑1 (MQ=255) aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTc > 1:94453/1‑72 (MQ=255) gCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTgg > 2:854258/1‑139 (MQ=255) ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggc > 1:110787/1‑139 (MQ=255) ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggc > 2:1030334/1‑139 (MQ=255) | GCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGC > NC_000913/309230‑309492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |