Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,330,396 G→A *478* (TAG→TAA dacB → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,3960GA100.0% 92.8 / NA 30*478* (TAG→TAAdacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

AGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330261‑3330531
                                                                                                                                        |                                                                                                                                       
agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATc                                                                                                                                       <  2:602613/139‑1 (MQ=255)
agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATc                                                                                                                                       >  2:23361/1‑139 (MQ=255)
 gcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCa                                                                                                                                      >  2:157624/1‑139 (MQ=255)
    agcgGGCAACGAATGGCGTTTGTGCAATACCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGAATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAAATATTTATCAGAACAATTAATCaaaa                                                                                                                                   >  1:743132/1‑139 (MQ=255)
       gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                                <  2:1030346/139‑1 (MQ=255)
                   gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcaca                                                                                                                    >  2:683854/1‑139 (MQ=255)
                    cGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACAt                                                                                                                   >  1:28937/1‑139 (MQ=255)
                         gtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                              >  1:99253/1‑139 (MQ=255)
                          tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATc                                                                                                                                       <  2:855782/113‑1 (MQ=255)
                          tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATc                                                                                                                                       >  1:855782/1‑113 (MQ=255)
                                atCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                                                       <  2:99253/139‑1 (MQ=255)
                                                aGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGt                                                                                       <  2:28937/139‑1 (MQ=255)
                                                     aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                              >  1:41003/1‑111 (MQ=255)
                                                     aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                              <  2:41003/111‑1 (MQ=255)
                                                     aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                                                  >  1:989655/1‑139 (MQ=255)
                                                       ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt                                                                                >  1:413244/1‑139 (MQ=255)
                                                                        tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTCGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatt                                                                                                  <  1:693087/104‑1 (MQ=255)
                                                                        ccGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatt                                                                                                  >  2:693087/2‑104 (MQ=255)
                                                                             gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                          <  2:45033/139‑1 (MQ=255)
                                                                                               tttGTAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                              <  2:411562/101‑1 (MQ=255)
                                                                                               tttGTAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                              >  1:411562/1‑101 (MQ=255)
                                                                                                         gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                              <  2:413244/139‑1 (MQ=255)
                                                                                                                   aGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                    <  2:38236/139‑1 (MQ=255)
                                                                                                                     atatTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata                  <  2:330625/139‑1 (MQ=255)
                                                                                                                               gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                                                  <  2:52741/65‑1 (MQ=255)
                                                                                                                               gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                                                  >  1:52741/1‑65 (MQ=255)
                                                                                                                               gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtct        >  1:1111549/1‑139 (MQ=255)
                                                                                                                                     ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  <  2:511879/139‑1 (MQ=255)
                                                                                                                                     ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  >  1:511879/1‑139 (MQ=255)
                                                                                                                                     ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  <  1:23361/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330261‑3330531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: