Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,373	G→C	intergenic (‑2/+4)	caiE ← / ← caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,373	0	G	C	100.0%	174.8 / NA	49	intergenic (‑2/+4)	caiE/caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/28);  total (21/28)

ACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGAT  >  NC_000913/35240‑35495                                                                                                                                      |                                                                                                                           acGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGcttgtt                                                                                                                       <  2:83974/139‑1 (MQ=255)      gTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                  >  2:152444/1‑139 (MQ=255)       tGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCCATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTc                                                                                                                 >  1:582024/1‑139 (MQ=255)           gCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                             <  2:223045/139‑1 (MQ=255)                tGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCcaca                                                                                                        <  2:703725/139‑1 (MQ=255)                tGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCcaca                                                                                                        >  2:331231/1‑139 (MQ=255)                tGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCcaca                                                                                                        <  2:298620/139‑1 (MQ=255)                          tAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATcgcg                                                                                              <  1:611160/139‑1 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCt                                                                                                               <  1:1069740/115‑1 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCt                                                                                                               >  2:1069740/1‑115 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                       >  1:13498/1‑139 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                       >  1:800645/1‑139 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                       >  1:612297/1‑139 (MQ=255)                                   gCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGc                                                                                     >  1:763380/1‑139 (MQ=255)                                    cTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCa                                                                                    >  1:135041/1‑139 (MQ=255)                                            aTCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAg                                                                            <  1:1114509/139‑1 (MQ=255)                                                   cGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCt                                                                     >  2:250468/1‑139 (MQ=255)                                                       aaTCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCaa                                                                 <  2:566445/139‑1 (MQ=255)                                                        aTCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAAt                                                                <  1:448845/139‑1 (MQ=255)                                                                  cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                      >  2:630673/1‑139 (MQ=255)                                                                  cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                      <  2:486400/139‑1 (MQ=255)                                                                       ggATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                           >  1:1079581/1‑113 (MQ=255)                                                                       ggATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                           <  2:1079581/113‑1 (MQ=255)                                                                         aTGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCa                                               <  1:152444/139‑1 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                            >  1:925348/1‑139 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                            >  1:235023/1‑139 (MQ=255)                                                                             aCAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAAc                                           <  1:331231/139‑1 (MQ=255)                                                                              cAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAAcc                                          <  2:359955/139‑1 (MQ=255)                                                                                    ccGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGa                                    <  2:665372/139‑1 (MQ=255)                                                                                    ccGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGa                                    <  2:800645/139‑1 (MQ=255)                                                                                     cGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                   <  2:135041/139‑1 (MQ=255)                                                                                     cGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                   <  2:960320/139‑1 (MQ=255)                                                                                     cGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                   <  1:630673/139‑1 (MQ=255)                                                                                      gTCGGGTGATCCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                  <  2:763380/139‑1 (MQ=255)                                                                                       tCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAg                                 <  2:358155/139‑1 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAATGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                     <  2:13498/139‑1 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                     <  2:235023/139‑1 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                     >  2:442574/1‑139 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                     >  1:424005/1‑139 (MQ=255)                                                                                                          tAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGc              <  1:1017721/139‑1 (MQ=255)                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                  <  2:960410/112‑1 (MQ=255)                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                  >  1:960410/1‑112 (MQ=255)                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAAtata       >  1:362442/1‑139 (MQ=255)                                                                                                                      ggCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGAt  <  2:564864/139‑1 (MQ=255)                                                                                                                      ggCCTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAACGGCATCTTCCGAATGCAGAACCGATAGATAGTGTTTCAACACGCCGCTGCGAATATAGCGAt  <  2:582024/139‑1 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                  <  1:90784/105‑1 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                  >  2:90784/1‑105 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTg               <  2:742758/124‑1 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTGCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAACGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTg               >  1:742758/1‑124 (MQ=255)                                                                                                                                 tCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGc                                                             >  2:597417/1‑69 (MQ=255)                                                                                                                                      |                                                                                                                           ACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGAT  >  NC_000913/35240‑35495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑