Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,170 G→A Q84* (CAG→TAG)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,1700GA100.0% 128.1 / NA 40Q84* (CAG→TAG) fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

CCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACG  >  NC_000913/3477037‑3477301
                                                                                                                                     |                                                                                                                                   
ccTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAacac                                                                                                                                <  1:72350/139‑1 (MQ=255)
    ttGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGc                                                                                                                            >  2:328457/1‑139 (MQ=255)
                                      cGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCt                                                                                                                    <  2:988454/113‑1 (MQ=255)
                                      cGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCt                                                                                                                    >  1:988454/1‑113 (MQ=255)
                                       gCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATGCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGtt                                                                                         >  1:663500/1‑139 (MQ=255)
                                            aGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGAt                                                                                                                         >  2:538131/1‑102 (MQ=255)
                                            aGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGAt                                                                                                                         <  1:538131/102‑1 (MQ=255)
                                                      ttCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTa                                                                          >  1:629808/1‑139 (MQ=255)
                                                      ttCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTa                                                                          >  2:906852/1‑139 (MQ=255)
                                                      ttCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTa                                                                          >  2:939768/1‑139 (MQ=255)
                                                        cACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTaga                                                                        <  2:629808/139‑1 (MQ=255)
                                                                cTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTc                                                                >  1:382650/1‑139 (MQ=255)
                                                                  tCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCa                                                              <  2:382650/139‑1 (MQ=255)
                                                                                  tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCt                                                                                                                    >  2:284355/1‑69 (MQ=255)
                                                                                  tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCt                                                                                                                    <  1:284355/69‑1 (MQ=255)
                                                                                  tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATc                                                                                                                >  2:896977/1‑73 (MQ=255)
                                                                                  tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATc                                                                                                                <  1:896977/73‑1 (MQ=255)
                                                                                  tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGa                                              >  2:128268/1‑139 (MQ=255)
                                                                                   cTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAg                                             >  1:266313/1‑139 (MQ=255)
                                                                                    tGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTagag                                                                       >  1:552140/1‑112 (MQ=255)
                                                                                    tGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTagag                                                                       <  2:552140/112‑1 (MQ=255)
                                                                                     gTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGTGTTTTCCATGTAACGACCCAGCGAGGc                                           <  2:663500/139‑1 (MQ=255)
                                                                                     gTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGc                                           <  1:51900/139‑1 (MQ=255)
                                                                                      ttCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGccc                                                                                             <  1:1089697/88‑1 (MQ=255)
                                                                                      ttCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGccc                                                                                             >  2:1089697/1‑88 (MQ=255)
                                                                                      ttCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGttttt                                                                                      >  1:384739/1‑95 (MQ=255)
                                                                                      ttCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGttttt                                                                                      <  2:384739/95‑1 (MQ=255)
                                                                                          aTCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGt                                                                                          <  2:575730/87‑1 (MQ=255)
                                                                                          aTCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGt                                                                                          >  1:575730/1‑87 (MQ=255)
                                                                                          aTCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCa                                      <  2:299679/139‑1 (MQ=255)
                                                                                              ctTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGc                                  >  2:406339/1‑139 (MQ=255)
                                                                                                tGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCAt                                >  2:61648/1‑139 (MQ=255)
                                                                                                   tCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAg                             <  2:180135/139‑1 (MQ=255)
                                                                                                                   tATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAg                             >  1:622770/1‑123 (MQ=255)
                                                                                                                   tATCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAg                             <  2:622770/123‑1 (MQ=255)
                                                                                                                    aTCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATg                                                                                                >  2:860394/1‑55 (MQ=255)
                                                                                                                    aTCAGCAAATGCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATg                                                                                                <  1:860394/55‑1 (MQ=255)
                                                                                                                              gCATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACg  <  1:906852/139‑1 (MQ=255)
                                                                                                                               cATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTaa                                                         >  2:660156/1‑83 (MQ=255)
                                                                                                                               cATCCTAAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTaa                                                         <  1:660156/83‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACG  >  NC_000913/3477037‑3477301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑