| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,569,410 | T→C | D637G (GAT→GGT) | glgX ← | glycogen debranching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,569,410 | 0 | T | C | 96.9% | 101.4 / ‑4.4 | 32 | D637G (GAT→GGT) | glgX | glycogen debranching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (15/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
TTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGC > NC_000913/3569298‑3569535 | ttAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCg < 1:967423/139‑1 (MQ=255) tGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCa < 2:776398/139‑1 (MQ=255) ctCGTCCTTCTCTAACCTAACCATGACTAACTCTTTTTTTATCACCTCTGGAACACACACAATCCGTGTGCAGGCCCCTGCCAGACACCCGTAATCACTCGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCAcg < 2:234547/138‑2 (MQ=255) aTCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACt > 2:1108741/1‑139 (MQ=255) aCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCTGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATg < 2:703254/114‑1 (MQ=255) aCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATg > 1:703254/1‑114 (MQ=255) aCCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATgg < 2:797903/111‑1 (MQ=255) aCCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATgg > 1:797903/1‑111 (MQ=255) tctcTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTa > 2:986007/1‑139 (MQ=255) acacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCAc > 2:799321/1‑82 (MQ=255) acacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCAc < 1:799321/82‑1 (MQ=255) aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGGATGGCGTGCCACTCCCcagcag > 2:1087827/1‑87 (MQ=255) aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGGATGGCGTGCCACTCCCcagcag < 1:1087827/87‑1 (MQ=255) aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCcagc > 1:597039/1‑85 (MQ=255) aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCcagc < 2:597039/85‑1 (MQ=255) cGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc < 1:1073081/71‑1 (MQ=255) cGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc > 2:1073081/1‑71 (MQ=255) tgCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCca > 2:1039343/1‑76 (MQ=255) tgCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCca < 1:1039343/76‑1 (MQ=255) cTGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAAtt < 2:377082/139‑1 (MQ=255) tGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAAttt > 2:254725/1‑139 (MQ=255) tGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAAtt < 1:837127/138‑1 (MQ=255) tGCCAGACAGCCGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAAtt > 2:837127/1‑138 (MQ=255) ccGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc > 2:573411/1‑47 (MQ=255) ccGTAATCACTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc < 1:573411/47‑1 (MQ=255) ccgcccTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGc < 1:694441/134‑1 (MQ=255) aCTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGt < 2:917372/85‑1 (MQ=255) aCTGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGt > 1:917372/1‑85 (MQ=255) tGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc > 2:382874/1‑37 (MQ=255) tGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGc < 1:382874/37‑1 (MQ=255) tGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGt > 1:316688/1‑78 (MQ=255) tGGGTTACCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGt < 2:316688/78‑1 (MQ=255) | TTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGC > NC_000913/3569298‑3569535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |