Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,643,899	G→A	G428G (GGC→GGT)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,643,899	0	G	A	100.0%	198.2 / NA	61	G428G (GGC→GGT)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

GACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGG  >  NC_000913/3643766‑3644018                                                                                                                                      |                                                                                                                        gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGATGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTAc                                                                                                                    >  2:626470/1‑139 (MQ=255)  aCCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACg                                                                                                                   <  1:538038/139‑1 (MQ=255)  aCCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACg                                                                                                                   <  2:509606/139‑1 (MQ=255)   ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGc                                                                                                                  <  1:227265/139‑1 (MQ=255)      aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGt                                                                                                               <  2:36649/139‑1 (MQ=255)      aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGt                                                                                                               >  1:271127/1‑139 (MQ=255)                    ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGcc                                                                                                 >  2:337960/1‑139 (MQ=255)                      tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTca                                                                                                             >  2:522338/1‑125 (MQ=255)                      tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTca                                                                                                             <  1:522338/125‑1 (MQ=255)                        aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCccgc                                                                                             >  1:147381/1‑139 (MQ=255)                         tCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCccgcc                                                                                            >  1:162954/1‑139 (MQ=255)                         tCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGAAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCccgcc                                                                                            >  2:175130/1‑139 (MQ=255)                            cTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACATGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCcgcg                                                                                         <  1:590674/139‑1 (MQ=255)                            cTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCcgcg                                                                                         >  1:562210/1‑139 (MQ=255)                              tcgtcgTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                       >  2:502103/1‑139 (MQ=255)                              tcgtcgTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                       >  1:366072/1‑139 (MQ=255)                               cgtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCtt                                                                                      >  2:937224/1‑139 (MQ=255)                                  cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtt                                                                                   >  1:999120/1‑139 (MQ=255)                                    tgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                 <  1:758200/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  2:493525/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  2:271127/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  2:562210/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  2:714334/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  1:941713/139‑1 (MQ=255)                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  1:937224/139‑1 (MQ=255)                                         gaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACgg                                                                            <  2:999120/139‑1 (MQ=255)                                         gaACAGCGCCGGTTTACCATATACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCAcag                                                                            >  2:653032/1‑137 (MQ=255)                                          aaCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACggg                                                                           >  2:563489/1‑139 (MQ=255)                                          aaCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACggg                                                                           >  2:728776/1‑139 (MQ=255)                                                 ccGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCcgcg                                                                                         >  1:907082/1‑118 (MQ=255)                                                 ccGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCcgcg                                                                                         <  2:907082/118‑1 (MQ=255)                                                 ccGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATTCAg                                                                    >  1:269301/1‑139 (MQ=255)                                                         ccATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGt                                                            <  2:162954/139‑1 (MQ=255)                                                             ttACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGaa                                                        >  1:357842/1‑139 (MQ=255)                                                              tACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACACCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAg                                                       >  2:149594/1‑139 (MQ=255)                                                                 cGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGc                                                                                                                  >  2:574976/1‑77 (MQ=255)                                                                 cGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGc                                                                                                                  <  1:574976/77‑1 (MQ=255)                                                                   ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCccg                                                                                              >  2:1101942/1‑95 (MQ=255)                                                                   ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCccg                                                                                              <  1:1101942/95‑1 (MQ=255)                                                                   ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                  <  1:561865/123‑1 (MQ=255)                                                                   ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                  >  2:561865/1‑123 (MQ=255)                                                                    gCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                 >  1:1086049/1‑139 (MQ=255)                                                                      ggTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                               <  2:147381/139‑1 (MQ=255)                                                                      ggTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGAAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                               <  1:175130/139‑1 (MQ=255)                                                                       gTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCgcgc                                              <  1:471529/139‑1 (MQ=255)                                                                                 cAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                    <  1:563489/139‑1 (MQ=255)                                                                                  aaGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAtt                                   >  2:48378/1‑139 (MQ=255)                                                                                   aGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAttt                                  <  1:368971/139‑1 (MQ=255)                                                                                      cAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCg                               >  1:780731/1‑139 (MQ=255)                                                                                           aCGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                          <  2:761233/139‑1 (MQ=255)                                                                                              cgACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTcat                                                                                                            <  1:268358/54‑1 (MQ=255)                                                                                              cgACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTcat                                                                                                            >  2:268358/1‑54 (MQ=255)                                                                                                  cGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATTCAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCg                   <  2:269301/139‑1 (MQ=255)                                                                                                             aaGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTAc        <  1:337960/139‑1 (MQ=255)                                                                                                                   cATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCgg  <  1:922562/139‑1 (MQ=255)                                                                                                                             aCGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                  <  2:1017439/65‑1 (MQ=255)                                                                                                                             aCGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                  >  1:1017439/1‑65 (MQ=255)                                                                                                                              cGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             >  1:289142/1‑53 (MQ=255)                                                                                                                              cGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                             <  2:289142/53‑1 (MQ=255)                                                                                                                                   cTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                    <  2:558679/89‑1 (MQ=255)                                                                                                                                   cTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                    >  1:558679/1‑89 (MQ=255)                                                                                                                                      |                                                                                                                        GACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGG  >  NC_000913/3643766‑3644018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑