Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,658,893 | G→A | *176* (TAG→TAA) | gadE → | gad regulon transcriptional activator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,658,893 | 0 | G | A | 100.0% | 238.6 / NA | 73 | *176* (TAG→TAA) | gadE | gad regulon transcriptional activator |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/39); total (34/39) |
AAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAC > NC_000913/3658757‑3659027 | aaaatGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATc < 2:240604/139‑1 (MQ=255) atCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAAc < 1:395697/139‑1 (MQ=255) cGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGaa > 2:961991/1‑139 (MQ=255) cGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATc < 1:1095600/118‑1 (MQ=255) cGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAAtt > 2:811795/1‑139 (MQ=255) cAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggct < 2:372388/139‑1 (MQ=255) cAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggct < 1:811795/139‑1 (MQ=255) ttCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg > 2:214684/1‑139 (MQ=255) gCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg < 1:574786/139‑1 (MQ=255) aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAg > 2:418719/1‑139 (MQ=255) aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAg > 2:91606/1‑139 (MQ=255) aaaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc > 1:669051/1‑121 (MQ=255) aaaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc < 2:669051/121‑1 (MQ=255) aaaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGAt > 2:879749/1‑139 (MQ=255) gATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCt < 1:563176/139‑1 (MQ=255) ttCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa < 1:879749/139‑1 (MQ=255) tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt > 2:327958/1‑139 (MQ=255) cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGAt > 2:353384/1‑132 (MQ=255) cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGAt < 1:353384/132‑1 (MQ=255) cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAtt > 1:560869/1‑139 (MQ=255) cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTACCACATCTTATTTTTAATCAGGACATAAGCAAc < 2:850050/97‑1 (MQ=255) cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTACCACATCTTATTTTTAATCAGGACATAAGCAAc > 1:850050/1‑97 (MQ=255) aTTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt < 1:961991/139‑1 (MQ=255) aCATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc < 2:560869/139‑1 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc < 2:525125/103‑1 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc > 1:525125/1‑103 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa < 1:91606/139‑1 (MQ=255) gTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt < 2:417640/139‑1 (MQ=255) aGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa > 1:1070210/1‑139 (MQ=255) gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa > 2:194053/1‑139 (MQ=255) aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga < 1:194053/139‑1 (MQ=255) aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga > 1:759090/1‑139 (MQ=255) aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga < 1:978987/139‑1 (MQ=255) aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATa < 2:534010/63‑1 (MQ=255) aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATa > 1:534010/1‑63 (MQ=255) aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTc < 1:212823/113‑1 (MQ=255) aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTc > 2:212823/1‑113 (MQ=255) gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt > 1:860990/1‑104 (MQ=255) gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt < 2:860990/104‑1 (MQ=255) gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTa > 1:590376/1‑139 (MQ=255) gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg > 2:1008317/1‑83 (MQ=255) gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACg < 1:1008317/83‑1 (MQ=255) aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg < 1:1067497/115‑1 (MQ=255) aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg > 2:1067497/1‑115 (MQ=255) aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTc > 2:561497/1‑139 (MQ=255) aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTc < 1:176789/139‑1 (MQ=255) tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCa < 2:392410/93‑1 (MQ=255) tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCa > 1:392410/1‑93 (MQ=255) gTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc < 1:561497/139‑1 (MQ=255) tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt < 2:11193/104‑1 (MQ=255) tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt > 1:11193/1‑104 (MQ=255) tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga > 2:965092/1‑114 (MQ=255) tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga < 1:965092/114‑1 (MQ=255) tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCt > 2:123932/1‑139 (MQ=255) cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt < 2:759090/139‑1 (MQ=255) cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt < 1:418719/139‑1 (MQ=255) ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg > 2:365407/1‑49 (MQ=255) ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg < 1:365407/49‑1 (MQ=255) gTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACTAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa < 1:173438/123‑1 (MQ=255) gTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa > 2:173438/1‑123 (MQ=255) tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa < 1:214684/139‑1 (MQ=255) tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa > 2:221031/1‑139 (MQ=255) tCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATc < 1:123932/139‑1 (MQ=255) cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATcc < 2:590376/139‑1 (MQ=255) cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATcc < 2:1070210/139‑1 (MQ=255) acaTCTTATATTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATcca > 2:82232/1‑139 (MQ=255) caTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATccac < 1:417926/139‑1 (MQ=255) aTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg < 2:1098547/49‑1 (MQ=255) aTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg > 1:1098547/1‑49 (MQ=255) cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc < 2:1000117/49‑1 (MQ=255) cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc > 1:1000117/1‑49 (MQ=255) cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg > 1:998465/1‑139 (MQ=255) tttAATCAGGACATAAGCAACTGAAATTGATGGCTCGCCTGACGAGGGATGCAGATGCTGATTTCATTACCCGCGGTGATTACTAAAGGCGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAc > 2:1062417/1‑139 (MQ=255) | AAAATGAAAGCAATATCACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAC > NC_000913/3658757‑3659027 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |