Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,017	G→A	*397* (TAG→TAA)	lldD →	L‑lactate dehydrogenase, FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,017	0	G	A	100.0%	151.6 / NA	47	*397* (TAG→TAA)	lldD	L‑lactate dehydrogenase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

GATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGG  >  NC_000913/3780888‑3781147                                                                                                                                  |                                                                                                                                   gaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg                                                                                                                           <  1:538827/139‑1 (MQ=255) gaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg                                                                                                                           <  2:994413/139‑1 (MQ=255)     aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGcccc                                                                                                                       >  2:842433/1‑139 (MQ=255)       aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     <  1:392288/139‑1 (MQ=255)            cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCAccc                                                                                                                >  2:34620/1‑139 (MQ=255)                   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                         >  1:245670/1‑139 (MQ=255)                   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                         <  2:479783/139‑1 (MQ=255)                   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCct                                                                                                         >  1:525478/1‑139 (MQ=255)                     tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                       <  2:245670/139‑1 (MQ=255)                     tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                       <  1:842433/139‑1 (MQ=255)                            cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                >  2:901015/1‑139 (MQ=255)                             gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     <  2:528609/117‑1 (MQ=255)                             gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     >  1:528609/1‑117 (MQ=255)                              cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAggg                                                                                              <  2:280315/139‑1 (MQ=255)                                    cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                        <  2:473496/139‑1 (MQ=255)                                             aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                      >  1:62827/1‑132 (MQ=255)                                             aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                      <  2:62827/132‑1 (MQ=255)                                             aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                               <  1:262200/139‑1 (MQ=255)                                              aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                              <  1:224337/139‑1 (MQ=255)                                                ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                            <  1:787670/139‑1 (MQ=255)                                                ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                            <  2:1077202/139‑1 (MQ=255)                                                  aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                          <  2:525478/139‑1 (MQ=255)                                                                  tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     >  1:638782/1‑80 (MQ=255)                                                                  tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     <  2:638782/80‑1 (MQ=255)                                                                    cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     >  1:209654/1‑78 (MQ=255)                                                                    cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     <  2:209654/78‑1 (MQ=255)                                                                           tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCa                                                                                                                   >  1:192873/1‑73 (MQ=255)                                                                           tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCa                                                                                                                   <  2:192873/73‑1 (MQ=255)                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                              >  1:415827/1‑109 (MQ=255)                                                                            gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                              <  2:415827/109‑1 (MQ=255)                                                                             ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     <  2:796064/69‑1 (MQ=255)                                                                             ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt                                                                                                                     >  1:796064/1‑69 (MQ=255)                                                                                 aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg                                           >  1:574675/1‑139 (MQ=255)                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                      >  2:108841/1‑74 (MQ=255)                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                      <  1:108841/74‑1 (MQ=255)                                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActct                                     >  1:320515/1‑139 (MQ=255)                                                                                                       tCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                     <  2:710757/139‑1 (MQ=255)                                                                                                          cATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                             <  1:1114992/80‑1 (MQ=255)                                                                                                          cATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                             >  2:1114992/1‑80 (MQ=255)                                                                                                             ggCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg               >  1:984717/1‑139 (MQ=255)                                                                                                               cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg             >  1:418417/1‑139 (MQ=255)                                                                                                                gAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg            <  2:320515/139‑1 (MQ=255)                                                                                                                 aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                               >  2:474267/1‑55 (MQ=255)                                                                                                                 aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                               <  1:474267/55‑1 (MQ=255)                                                                                                                        aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                      <  2:756743/41‑1 (MQ=255)                                                                                                                        aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                      >  1:756743/1‑41 (MQ=255)                                                                                                                          gCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg  <  1:34620/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   GATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGG  >  NC_000913/3780888‑3781147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑