Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA
G430G (GGC→GGT
cbrA →
dgoT ←
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA100.0% 157.1 / NA 51*355* (TAG→TAA
G430G (GGC→GGT
cbrA
dgoT
colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/28);  total (23/28)

CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCAC  >  NC_000913/3870305‑3870560
                                                                                                                                        |                                                                                                                       
cTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAAcc                                                                                                                       >  2:1105708/1‑139 (MQ=255)
                        tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               <  1:644562/139‑1 (MQ=255)
                        tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGACTGAACGCACCGGCTTTAAGAAAGTAGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               <  1:1081306/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             <  1:1105708/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             <  1:57592/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             <  2:396491/139‑1 (MQ=255)
                                           cAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                            <  1:333433/139‑1 (MQ=255)
                                                tAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAAt                                                                       >  1:1011729/1‑139 (MQ=255)
                                                        aGCTGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCTCATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                               >  2:716136/1‑139 (MQ=255)
                                                         gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAc                                                              >  2:560529/1‑139 (MQ=255)
                                                          ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                             >  1:1080589/1‑139 (MQ=255)
                                                            gATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                             >  2:179009/1‑137 (MQ=255)
                                                            gATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                             <  1:179009/137‑1 (MQ=255)
                                                             aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                     >  2:629517/1‑96 (MQ=255)
                                                             aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                     <  1:629517/96‑1 (MQ=255)
                                                             aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcg                                                          <  2:1011729/139‑1 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         <  2:5703/139‑1 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         <  1:727159/139‑1 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         <  2:1080589/139‑1 (MQ=255)
                                                                        cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAAc                                               >  2:118303/1‑139 (MQ=255)
                                                                         aCCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             >  2:673672/1‑92 (MQ=255)
                                                                         aCCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             <  1:673672/92‑1 (MQ=255)
                                                                                   aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             <  1:454448/82‑1 (MQ=255)
                                                                                   aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                             >  2:454448/1‑82 (MQ=255)
                                                                                   aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCGTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTg                                    >  1:588265/1‑139 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTc                                                                                                             >  2:960229/1‑62 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTc                                                                                                             <  1:960229/62‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               >  1:33804/1‑76 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               <  2:33804/76‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                               <  1:752509/108‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATAAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                               >  2:752509/1‑108 (MQ=255)
                                                                                        gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACGTCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaaa                               >  1:157488/1‑139 (MQ=255)
                                                                                           gATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGCATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAACCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCg                            <  1:743829/139‑1 (MQ=255)
                                                                                           gATCATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCg                            <  1:716136/139‑1 (MQ=255)
                                                                                                     aGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTgcg                  <  1:300345/139‑1 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         >  2:356106/1‑95 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         <  1:356106/95‑1 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaa                                                 >  2:284652/1‑103 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaa                                                 <  1:284652/103‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                            <  2:654827/35‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                            >  1:654827/1‑35 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         <  2:454919/86‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                         >  1:454919/1‑86 (MQ=255)
                                                                                                                    atTCCACAGTTGAAGGATTAACCATCGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCAAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGCGCGAAACCGTAACCCTGCGCCAGGTACCccacca   >  2:790620/1‑139 (MQ=255)
                                                                                                                    atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAagaga                                                                                 >  1:404197/1‑61 (MQ=255)
                                                                                                                    atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAagaga                                                                                 <  2:404197/61‑1 (MQ=255)
                                                                                                                     ttCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccaccac  <  1:560529/139‑1 (MQ=255)
                                                                                                                         acaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               <  2:16720/42‑1 (MQ=255)
                                                                                                                         acaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               >  1:16720/1‑42 (MQ=255)
                                                                                                                              tGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               <  2:570225/37‑1 (MQ=255)
                                                                                                                              tGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                               >  1:570225/1‑37 (MQ=255)
                                                                                                                                        |                                                                                                                       
CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCAC  >  NC_000913/3870305‑3870560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: