Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,923,070 G→A A204V (GCA→GTA)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,0700GA100.0% 54.1 / NA 18A204V (GCA→GTA) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

AGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTT  >  NC_000913/3922958‑3923188
                                                                                                                |                                                                                                                      
agTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                              <  2:356061/139‑1 (MQ=255)
  tGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   >  2:773177/1‑132 (MQ=255)
  tGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   <  1:773177/132‑1 (MQ=255)
                           ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAAcc                                                                            <  1:303396/130‑1 (MQ=255)
                           ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAAcc                                                                            >  2:303396/1‑130 (MQ=255)
                                     aGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcacc                                                                                                             <  2:924636/87‑1 (MQ=255)
                                     aGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcacc                                                                                                             >  1:924636/1‑87 (MQ=255)
                                     aGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                         >  2:761503/1‑139 (MQ=255)
                                     aGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                         >  1:953734/1‑139 (MQ=255)
                                       aaaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCTGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                       >  2:65135/1‑139 (MQ=255)
                                          aCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAcc                                                    <  1:470617/139‑1 (MQ=255)
                                              ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   <  2:256661/88‑1 (MQ=255)
                                              ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   <  2:603318/88‑1 (MQ=255)
                                              ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   >  1:256661/1‑88 (MQ=255)
                                              ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGAc                                                                                                   >  1:603318/1‑88 (MQ=255)
                                                                                aTGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                              <  2:261004/59‑1 (MQ=255)
                                                                                aTGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                              >  1:261004/1‑59 (MQ=255)
                                                                                            aTAAAAATTAAATTTTATTTACTTTAATCACCACCTGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCttt  <  1:65135/139‑1 (MQ=255)
                                                                                                                |                                                                                                                      
AGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTT  >  NC_000913/3922958‑3923188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: