Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,168	G→A	*297* (TAG→TAA)	rbsB →	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,168	0	G	A	100.0%	143.4 / NA	46	*297* (TAG→TAA)	rbsB	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

CTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGA  >  NC_000913/3937037‑3937291                                                                                                                                    |                                                                                                                            cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                      <  2:986265/139‑1 (MQ=255)          aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                             >  2:35715/1‑139 (MQ=255)          aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                             >  2:252269/1‑139 (MQ=255)          aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                             <  2:172016/139‑1 (MQ=255)          aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                             <  1:35715/139‑1 (MQ=255)                         cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                              >  1:44922/1‑139 (MQ=255)                                aGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATa                                                                                       >  2:638681/1‑139 (MQ=255)                                  aTTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                     <  2:954281/139‑1 (MQ=255)                                  aTTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                     <  1:632998/139‑1 (MQ=255)                                    tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                   <  2:44922/139‑1 (MQ=255)                                    tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                   >  1:1014329/1‑139 (MQ=255)                                      gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                                 <  1:328249/139‑1 (MQ=255)                                                cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                       <  1:638681/139‑1 (MQ=255)                                                  tCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                   >  1:368368/1‑93 (MQ=255)                                                  tCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                   <  2:368368/93‑1 (MQ=255)                                                                        gAAAGGCGAGAAAGTTCAGGCTTAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACTTGATGGTGACATAAATACGTCAt                                                                                 >  1:997303/1‑105 (MQ=255)                                                                        gAAAGGCGAGAAAGTTCAGGCTTAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                                 <  2:997303/105‑1 (MQ=255)                                                                           aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgt                                                                   >  1:966255/1‑116 (MQ=255)                                                                           aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgt                                                                   <  2:966255/116‑1 (MQ=255)                                                                            ggCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                   >  1:866336/1‑99 (MQ=255)                                                                            ggCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                   <  2:866336/99‑1 (MQ=255)                                                                              cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                     <  2:277172/95‑1 (MQ=255)                                                                              cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                     >  1:277172/1‑95 (MQ=255)                                                                              cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGAGTAATATAAAGAAAAGCAGGGCAcgc                                         >  1:302824/1‑139 (MQ=255)                                                                                            cTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                  >  1:680381/1‑100 (MQ=255)                                                                                            cTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                  <  2:680381/100‑1 (MQ=255)                                                                                                gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAgg                                                                                                                  >  1:341857/1‑48 (MQ=255)                                                                                                gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAgg                                                                                                                  <  2:341857/48‑1 (MQ=255)                                                                                                gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                              <  1:865354/68‑1 (MQ=255)                                                                                                gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                              >  2:865354/1‑68 (MQ=255)                                                                                                     cGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                  >  1:885636/1‑139 (MQ=255)                                                                                                       gTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                       <  2:1111178/84‑1 (MQ=255)                                                                                                       gTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                       >  1:1111178/1‑84 (MQ=255)                                                                                                         tGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                  >  2:519404/1‑87 (MQ=255)                                                                                                         tGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                  <  1:519404/87‑1 (MQ=255)                                                                                                          gATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTATAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTAt             >  2:519104/1‑139 (MQ=255)                                                                                                           aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGAGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATg            <  2:302824/139‑1 (MQ=255)                                                                                                              tGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGAc         <  2:1014329/139‑1 (MQ=255)                                                                                                              tGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGAc         <  1:519104/139‑1 (MQ=255)                                                                                                                     ggTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGa  >  2:346321/1‑139 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcg                                          >  2:112808/1‑98 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcg                                          <  1:112808/98‑1 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                       >  2:279021/1‑101 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                       <  1:279021/101‑1 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcgc                    <  2:162496/120‑1 (MQ=255)                                                                                                                      gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcgc                    >  1:162496/1‑120 (MQ=255)                                                                                                                                    |                                                                                                                            CTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGA  >  NC_000913/3937037‑3937291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑