Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,961,562 C→T H296Y (CAC→TAC)  rep → DNA helicase and single‑stranded DNA‑dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,961,5620CT100.0% 106.5 / NA 34H296Y (CAC→TAC) repDNA helicase and single‑stranded DNA‑dependent ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

GCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACG  >  NC_000913/3961426‑3961696
                                                                                                                                        |                                                                                                                                      
gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTAc                                                                                                                                      >  2:831686/1‑139 (MQ=255)
gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTAc                                                                                                                                      >  2:280205/1‑139 (MQ=255)
gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTAc                                                                                                                                      >  2:178988/1‑139 (MQ=255)
gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAAATATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTAc                                                                                                                                      >  1:11833/1‑139 (MQ=255)
 cgcgGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACg                                                                                                                                     <  1:1017902/139‑1 (MQ=255)
          cGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGCTTAAGCTTGAGCAGAACTATCGCGCTTCCGGGCGTATTCAGAAAGCGGCGTACATCCTGATCGCCAATAACCCGTACGTCTTTGaaa                                                                                                                            <  1:707310/139‑1 (MQ=255)
          cGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGaaa                                                                                                                            <  1:831686/139‑1 (MQ=255)
                     ccTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTtc                                                                                                                 >  1:829758/1‑139 (MQ=255)
                        ggTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTTTCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTcc                                                                                                              >  2:757863/1‑139 (MQ=255)
                                    tCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTAt                                                                                                  >  1:961892/1‑139 (MQ=255)
                                        gATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcg                                                                                              <  1:686501/139‑1 (MQ=255)
                                              ccGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGc                                                                                        <  1:280205/139‑1 (MQ=255)
                                                 gcgcTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTaa                                                                                     <  1:398415/139‑1 (MQ=255)
                                                                    ttGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATaa                                                                  <  1:178988/139‑1 (MQ=255)
                                                                       aGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGaa                                                                                                           >  1:450269/1‑95 (MQ=255)
                                                                       aGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGaa                                                                                                           <  2:450269/95‑1 (MQ=255)
                                                                       aGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcgc                                                                                             >  2:921372/1‑109 (MQ=255)
                                                                       aGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcgc                                                                                             <  1:921372/109‑1 (MQ=255)
                                                                        gCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACgaa                                                              <  2:120770/139‑1 (MQ=255)
                                                                               tATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATg                                                       <  1:906844/139‑1 (MQ=255)
                                                                                 tCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCt                                                                                                                     <  1:412290/75‑1 (MQ=255)
                                                                                 tCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCt                                                                                                                     >  2:412290/1‑75 (MQ=255)
                                                                                 tCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAg                                                     >  2:569299/1‑139 (MQ=255)
                                                                                       ttCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAg                                               <  2:829758/139‑1 (MQ=255)
                                                                                           gggCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGaaa                                                                                                                            >  2:196445/1‑58 (MQ=255)
                                                                                           gggCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGaaa                                                                                                                            <  1:196445/58‑1 (MQ=255)
                                                                                           gggCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGcgcg                                           >  1:253330/1‑139 (MQ=255)
                                                                                                 aTTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGc                                                   <  2:68973/125‑1 (MQ=255)
                                                                                                 aTTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGc                                                   >  1:68973/1‑125 (MQ=255)
                                                                                                       aaaGCGGCGAACATCCTGATCGCCAATAACCCGTACg                                                                                                                                     <  1:979623/37‑1 (MQ=255)
                                                                                                       aaaGCGGCGAACATCCTGATCGCCAATAACCCGTACg                                                                                                                                     >  2:979623/1‑37 (MQ=255)
                                                                                                            ggcgAACATCCTGATCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATc                          >  2:668622/1‑139 (MQ=255)
                                                                                                                          tCGCCAATAACCCGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGt            <  1:757863/139‑1 (MQ=255)
                                                                                                                                    cccGTACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACg  <  2:11833/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTATTAAGCGCGAATAACGAAGAACATGAGGCTGAGCGCGTTACTGGCGAGCTGATCGCCCATCACTTCGTCAATAAAACG  >  NC_000913/3961426‑3961696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑