Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,693	G→A	*610* (TAG→TAA)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,693	0	G	A	100.0%	114.1 / NA	36	*610* (TAG→TAA)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

ATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGT  >  NC_000913/4007559‑4007816                                                                                                                                       |                                                                                                                            aTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                         <  2:778431/139‑1 (MQ=255) aTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                         <  2:1019936/139‑1 (MQ=255)   ggCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagca                                                                                                                       <  1:804010/139‑1 (MQ=255)              tGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAg                                                                                                            >  1:239448/1‑139 (MQ=255)               gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagcag                                                                                                                      >  2:934241/1‑128 (MQ=255)               gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagcag                                                                                                                      <  1:934241/128‑1 (MQ=255)                  gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                        >  2:982364/1‑139 (MQ=255)                   aTCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCa                                                                                                       >  2:967695/1‑139 (MQ=255)                       ccGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTAt                                                                                                   >  1:97135/1‑139 (MQ=255)                             gCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGAc                                                                                             >  2:879970/1‑139 (MQ=255)                                     cTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTaaa                                                                                     >  1:868969/1‑139 (MQ=255)                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                     <  2:869696/139‑1 (MQ=255)                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                     <  1:879970/139‑1 (MQ=255)                                                     ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                     <  1:982364/139‑1 (MQ=255)                                                          aTGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAat                                                                <  1:219071/139‑1 (MQ=255)                                                          aTGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAat                                                                <  2:239448/139‑1 (MQ=255)                                                             cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatgta                                                             <  2:682965/139‑1 (MQ=255)                                                                  gCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                       <  1:870582/108‑1 (MQ=255)                                                                  gCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                       >  2:870582/1‑108 (MQ=255)                                                                       aaCGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATg                                                     <  1:232084/137‑1 (MQ=255)                                                                       aaCGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATg                                                     >  2:232084/1‑137 (MQ=255)                                                                            tttGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                         <  2:237647/64‑1 (MQ=255)                                                                            tttGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                         >  1:237647/1‑64 (MQ=255)                                                                                gCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCAc                                          <  1:784294/139‑1 (MQ=255)                                                                                   aaCCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTGCCCCCGCGAGTCAAATGTATGTTGATGTTATTACTCACCGt                                       <  2:914849/139‑1 (MQ=255)                                                                                                gcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGcac                          >  2:625101/1‑139 (MQ=255)                                                                                                    aTTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATtg                      <  2:97135/139‑1 (MQ=255)                                                                                                              aTGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTAt            >  2:318047/1‑139 (MQ=255)                                                                                                              aTGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTAt            >  2:308375/1‑139 (MQ=255)                                                                                                                        cGATGACTAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                        <  2:642464/37‑1 (MQ=255)                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                        >  1:642464/1‑37 (MQ=255)                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAGGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCa                                  <  2:948766/107‑1 (MQ=255)                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAGGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCa                                  >  1:948766/1‑107 (MQ=255)                                                                                                                        cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGATATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGt  >  1:527538/1‑139 (MQ=255)                                                                                                                          aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGa                                                                       >  1:186181/1‑68 (MQ=255)                                                                                                                          aTGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGa                                                                       <  2:186181/68‑1 (MQ=255)                                                                                                                                       |                                                                                                                            ATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGT  >  NC_000913/4007559‑4007816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑