Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,024,336 G→A *261* (TAG→TAA
L162L (CTC→CTT
tatD →
rfaH ←
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,024,3360GA100.0% 178.1 / NA 55*261* (TAG→TAA
L162L (CTC→CTT
tatD
rfaH
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

ATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTT  >  NC_000913/4024201‑4024463
                                                                                                                                       |                                                                                                                                  
atcatcCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGt                                                                                                                                 <  1:248322/139‑1 (MQ=255)
        ggCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAAATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGCTTGCGGaa                                                                                                                         >  1:661289/1‑139 (MQ=255)
         gcgcAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCTACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAAc                                                                                                                        >  1:451696/1‑139 (MQ=255)
          cgcAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGATTGGGATTGCGTTTTAAAGTTTGCGGAACt                                                                                                                       >  2:94733/1‑139 (MQ=255)
            cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                     <  1:535181/139‑1 (MQ=255)
              aCGAGCCAGCCCATCTGCCCCATACTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  2:880787/139‑1 (MQ=255)
               cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTg                                                                                                                              >  2:571201/1‑127 (MQ=255)
               cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTg                                                                                                                              <  1:571201/127‑1 (MQ=255)
                  gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑ttc                                                                                                            <  2:866941/139‑1 (MQ=255)
                       cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                            >  1:192395/1‑121 (MQ=255)
                       cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                            <  2:192395/121‑1 (MQ=255)
                         cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCacac                                                                                                     <  2:927224/139‑1 (MQ=255)
                         cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCacac                                                                                                     <  2:661289/139‑1 (MQ=255)
                          aTCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACt                                                                                                    <  1:1014178/139‑1 (MQ=255)
                            cTGCCCCATATTTTGCAACGTATTGCGCAGTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACtgt                                                                                                  >  1:743432/1‑139 (MQ=255)
                            cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACtgt                                                                                                  >  2:332664/1‑139 (MQ=255)
                                 ccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCTACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTa                                                                                             <  2:451696/139‑1 (MQ=255)
                                   atatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAAc                                                                                                                        <  2:975955/113‑1 (MQ=255)
                                   atatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAAc                                                                                                                        >  1:975955/1‑113 (MQ=255)
                                    tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAAtc                                                                                          <  1:332664/139‑1 (MQ=255)
                                    tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGATTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAAtc                                                                                          <  1:94733/139‑1 (MQ=255)
                                              cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTcaca                                                                                                      >  1:463842/1‑117 (MQ=255)
                                              cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTcaca                                                                                                      <  2:463842/117‑1 (MQ=255)
                                              cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                >  2:955087/1‑139 (MQ=255)
                                              cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                >  2:504332/1‑139 (MQ=255)
                                               gTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                                          >  2:335073/1‑99 (MQ=255)
                                               gTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                                          <  1:335073/99‑1 (MQ=255)
                                               gTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                               <  1:504332/139‑1 (MQ=255)
                                                tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                <  1:434009/137‑1 (MQ=255)
                                                tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                >  2:434009/1‑137 (MQ=255)
                                                   tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                           <  1:955087/139‑1 (MQ=255)
                                                     cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACtgt                                                                                                  <  2:497728/114‑1 (MQ=255)
                                                     cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACtgt                                                                                                  >  1:497728/1‑114 (MQ=255)
                                                            gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCacac                                                                                                     <  1:1076115/104‑1 (MQ=255)
                                                            gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCacac                                                                                                     >  2:1076115/1‑104 (MQ=255)
                                                                    aagaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTCTGGGATTGCGTTTCAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGga                                                          <  2:743432/139‑1 (MQ=255)
                                                                      gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                <  2:330209/115‑1 (MQ=255)
                                                                      gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                >  1:330209/1‑115 (MQ=255)
                                                                           cGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTcaca                                                                                                      <  2:418341/88‑1 (MQ=255)
                                                                           cGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTcaca                                                                                                      >  1:418341/1‑88 (MQ=255)
                                                                           cGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                         <  2:556570/133‑1 (MQ=255)
                                                                           cGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                         >  1:556570/1‑133 (MQ=255)
                                                                                 gctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCa                                             >  2:142594/1‑139 (MQ=255)
                                                                                               cGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                              <  2:387161/108‑1 (MQ=255)
                                                                                               cGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                              >  1:387161/1‑108 (MQ=255)
                                                                                                    gCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATgg                          >  2:329926/1‑139 (MQ=255)
                                                                                                         tGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                     <  1:329926/139‑1 (MQ=255)
                                                                                                             aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   >  2:862458/1‑44 (MQ=255)
                                                                                                             aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                   <  1:862458/44‑1 (MQ=255)
                                                                                                                acacTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGc                 >  2:980563/1‑139 (MQ=255)
                                                                                                                     gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                >  2:251203/1‑68 (MQ=255)
                                                                                                                     gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                <  1:251203/68‑1 (MQ=255)
                                                                                                                            aTTGCGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt  <  2:961730/139‑1 (MQ=255)
                                                                                                                                cGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                         <  1:897879/80‑1 (MQ=255)
                                                                                                                                cGTTTTAAAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                         >  2:897879/1‑80 (MQ=255)
                                                                                                                                       |                                                                                                                                  
ATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTA‑‑‑TTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTT  >  NC_000913/4024201‑4024463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: