Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,681	C→T	G529S (GGC→AGC)	eptC ←	LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,681	0	C	T	100.0%	264.0 / NA	81	G529S (GGC→AGC)	eptC	LPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/43);  total (38/43)

CCTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAC  >  NC_000913/4148546‑4148815                                                                                                                                         |                                                                                                                                       ccTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     >  2:173857/1‑139 (MQ=255)            tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  1:510377/139‑1 (MQ=255)            tAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  1:486775/139‑1 (MQ=255)                gCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGgt                                                                                                                      <  1:127771/139‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  2:928280/132‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  1:928280/1‑132 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                                <  2:1058250/125‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                                >  1:1058250/1‑125 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATc                                                                                                                               <  1:405319/126‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATc                                                                                                                               >  2:405319/1‑126 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  2:659023/1‑131 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  1:659023/131‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           >  2:500077/1‑130 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           <  1:500077/130‑1 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  2:1004531/1‑139 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  1:684648/1‑139 (MQ=255)                    tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTAGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGACAAATCTGACCAGGTGTgg                                                                                                                  >  2:727557/1‑139 (MQ=255)                        tCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCa                                                                                                              >  2:1070487/1‑139 (MQ=255)                              tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     <  2:591186/110‑1 (MQ=255)                              tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     >  1:591186/1‑110 (MQ=255)                              tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCg                                                                                                        >  1:866647/1‑139 (MQ=255)                              tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGGGGATCAGTTCCg                                                                                                        >  2:458987/1‑139 (MQ=255)                                gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           >  1:444243/1‑118 (MQ=255)                                gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           <  2:444243/118‑1 (MQ=255)                                  tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                                    <  1:1070487/139‑1 (MQ=255)                                   cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                                   <  2:1110883/139‑1 (MQ=255)                                   cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                                   >  1:1110883/1‑139 (MQ=255)                                    aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  1:727557/139‑1 (MQ=255)                                          tttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  2:852068/1‑109 (MQ=255)                                          tttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  1:852068/109‑1 (MQ=255)                                          tttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTAtt                                                                                            >  2:18528/1‑139 (MQ=255)                                             ttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                                         <  2:570273/139‑1 (MQ=255)                                             ttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                                         <  2:675729/139‑1 (MQ=255)                                                ttATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGAt                                                                                      <  1:1022719/139‑1 (MQ=255)                                                     cGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  2:65062/122‑1 (MQ=255)                                                     cGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  >  1:65062/1‑122 (MQ=255)                                                        gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  1:284232/1‑95 (MQ=255)                                                        gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  2:284232/95‑1 (MQ=255)                                                         ttACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCt                                                                             >  2:809860/1‑139 (MQ=255)                                                          tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  1:862901/117‑1 (MQ=255)                                                          tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  >  2:862901/1‑117 (MQ=255)                                                             cAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCga                                                                         <  1:769525/139‑1 (MQ=255)                                                               aTCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCgaga                                                                       <  1:809860/139‑1 (MQ=255)                                                                  cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                                >  2:585170/1‑95 (MQ=255)                                                                  cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGAt                                                                                                                <  1:585170/95‑1 (MQ=255)                                                                  cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  >  2:43126/1‑109 (MQ=255)                                                                  cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  1:43126/109‑1 (MQ=255)                                                                    gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     >  2:65907/1‑72 (MQ=255)                                                                    gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     <  1:65907/72‑1 (MQ=255)                                                                    gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           >  2:391948/1‑82 (MQ=255)                                                                    gCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           <  1:391948/82‑1 (MQ=255)                                                                     cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCTCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCAc                                                                 >  2:624767/1‑139 (MQ=255)                                                                     cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCAc                                                                 >  2:36450/1‑139 (MQ=255)                                                                       ggTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                                  >  1:178707/1‑136 (MQ=255)                                                                       ggTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                                  <  2:178707/136‑1 (MQ=255)                                                                                   gAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCTCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCt                                                   <  1:624767/139‑1 (MQ=255)                                                                                    aaCTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                                <  1:56874/61‑1 (MQ=255)                                                                                    aaCTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAAt                                                                                                                                >  2:56874/1‑61 (MQ=255)                                                                                      cTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGc                                                <  1:173857/139‑1 (MQ=255)                                                                                      cTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGc                                                <  1:18528/139‑1 (MQ=255)                                                                                        gCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGc                                                                                                       >  2:895906/1‑82 (MQ=255)                                                                                        gCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGc                                                                                                       <  1:895906/82‑1 (MQ=255)                                                                                         cGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCAt                                             >  2:404279/1‑139 (MQ=255)                                                                                                       aaCGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     >  1:395801/1‑37 (MQ=255)                                                                                                       aaCGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGc                                                                                                                                     <  2:395801/37‑1 (MQ=255)                                                                                                       aaCGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCaa                                                                                                                                  >  1:358929/1‑40 (MQ=255)                                                                                                       aaCGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCaa                                                                                                                                  <  2:358929/40‑1 (MQ=255)                                                                                                            gTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  1:7435/43‑1 (MQ=255)                                                                                                            gTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  2:7435/1‑43 (MQ=255)                                                                                                            gTTGGATCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagca                          >  1:628713/1‑139 (MQ=255)                                                                                                                  tCGTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACg                    <  1:1004531/139‑1 (MQ=255)                                                                                                                    gTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                  <  2:628713/139‑1 (MQ=255)                                                                                                                    gTAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                  <  1:917156/139‑1 (MQ=255)                                                                                                                     tAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  2:684648/139‑1 (MQ=255)                                                                                                                     tAA‑CCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:404279/139‑1 (MQ=255)                                                                                                                             cgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                      >  2:823839/1‑47 (MQ=255)                                                                                                                             cgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                      <  1:823839/47‑1 (MQ=255)                                                                                                                                 aGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                                  <  2:120416/79‑1 (MQ=255)                                                                                                                                 aGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                                  >  1:120416/1‑79 (MQ=255)                                                                                                                                     aaGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAc  <  1:714574/139‑1 (MQ=255)                                                                                                                                      aGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCt                                                                                                 >  2:229020/1‑43 (MQ=255)                                                                                                                                         |                                                                                                                                       CCTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAA‑CCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAC  >  NC_000913/4148546‑4148815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑