Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,678	G→A	L222L (CTG→CTA)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,678	0	G	A	100.0%	103.1 / NA	33	L222L (CTG→CTA)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/14);  total (19/14)

TCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGG  >  NC_000913/4156557‑4156799                                                                                                                          |                                                                                                                          tCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                          >  2:891571/1‑139 (MQ=255)         aTTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTc                                                                                                  >  2:515490/1‑139 (MQ=255)                         ggCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTggg                                                                                                       <  1:411213/118‑1 (MQ=255)                         ggCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTggg                                                                                                       >  2:411213/1‑118 (MQ=255)                              cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                             >  2:161542/1‑139 (MQ=255)                                   tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCAt                                                                        <  1:515490/139‑1 (MQ=255)                                        aaaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGa                                                                   >  1:477385/1‑139 (MQ=255)                                        aaaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGa                                                                   >  1:960019/1‑139 (MQ=255)                                         aaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                 >  2:598991/1‑92 (MQ=255)                                         aaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                 <  1:598991/92‑1 (MQ=255)                                           tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGagca                                                                >  2:31507/1‑139 (MQ=255)                                                     gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  1:547519/139‑1 (MQ=255)                                                     gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  2:147091/139‑1 (MQ=255)                                                                 acTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                          <  2:29616/139‑1 (MQ=255)                                                                       tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCg                                    >  2:347278/1‑139 (MQ=255)                                                                        gAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGa                                   <  2:979146/139‑1 (MQ=255)                                                                                cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      >  2:422544/1‑112 (MQ=255)                                                                                cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  1:422544/112‑1 (MQ=255)                                                                                cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTAcg                           <  2:710542/139‑1 (MQ=255)                                                                                       aCTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGAttt                    <  2:84097/139‑1 (MQ=255)                                                                                          gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  1:840174/1‑139 (MQ=255)                                                                                          gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  2:342752/1‑139 (MQ=255)                                                                                          gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  1:211111/1‑139 (MQ=255)                                                                                           aCGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGc                <  1:891571/139‑1 (MQ=255)                                                                                             ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      >  1:609583/1‑99 (MQ=255)                                                                                             ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  2:609583/99‑1 (MQ=255)                                                                                               cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa            >  1:183571/1‑139 (MQ=255)                                                                                               cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATACCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATCCCGATGGATACGCGGATTTTCGCTTaa            >  2:727549/1‑139 (MQ=255)                                                                                                         aaagtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTgg  >  2:1059377/1‑139 (MQ=255)                                                                                                            gtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGttt                                                <  2:83706/90‑1 (MQ=255)                                                                                                            gtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGttt                                                >  1:83706/1‑90 (MQ=255)                                                                                                               aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGAt                                  <  1:436812/101‑1 (MQ=255)                                                                                                               aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGAt                                  >  2:436812/1‑101 (MQ=255)                                                                                                                          |                                                                                                                          TCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGG  >  NC_000913/4156557‑4156799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑