Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,213,680 C→T *148* (TAG→TAA)  yjaB ← GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,6800CT100.0% 178.5 / NA 56*148* (TAG→TAA) yjaBGNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACG  >  NC_000913/4213545‑4213809
                                                                                                                                       |                                                                                                                                 
ccAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGATAAAATCGCTTAc                                                                                                                                >  2:528117/1‑139 (MQ=255)
 cAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACg                                                                                                                               <  2:283459/139‑1 (MQ=255)
    ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                            <  2:494924/139‑1 (MQ=255)
             cATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                   >  1:428630/1‑139 (MQ=255)
               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                 >  1:842622/1‑139 (MQ=255)
               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                 >  1:266182/1‑139 (MQ=255)
                          cGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                      >  1:318393/1‑139 (MQ=255)
                                tCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATa                                                                                                >  2:239929/1‑139 (MQ=255)
                                  aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                      <  2:216888/131‑1 (MQ=255)
                                  aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                      >  1:216888/1‑131 (MQ=255)
                                    tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGt                                                                                            >  1:261276/1‑139 (MQ=255)
                                    tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGt                                                                                            <  2:738018/139‑1 (MQ=255)
                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcccc                                                                                      >  1:136039/1‑139 (MQ=255)
                                            cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaa                                                                                    >  1:524458/1‑139 (MQ=255)
                                             ggTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                   <  1:340673/139‑1 (MQ=255)
                                                 aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                 >  2:282785/1‑89 (MQ=255)
                                                  tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                              <  2:1101499/139‑1 (MQ=255)
                                                       aTGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGAtt                                                                                                            >  1:212660/1‑104 (MQ=255)
                                                       aTGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGAtt                                                                                                            <  2:212660/104‑1 (MQ=255)
                                                        tGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTc                                                                        <  1:692461/139‑1 (MQ=255)
                                                        tGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTc                                                                        >  1:865644/1‑139 (MQ=255)
                                                              aGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                        >  1:609582/1‑101 (MQ=255)
                                                              aGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                        <  2:609582/101‑1 (MQ=255)
                                                              aGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCg                                                                  >  2:352116/1‑139 (MQ=255)
                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCg                                                             >  1:807919/1‑139 (MQ=255)
                                                                         tCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                   <  2:958147/79‑1 (MQ=255)
                                                                         tCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                   >  1:958147/1‑79 (MQ=255)
                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaa                                                   >  1:614596/1‑139 (MQ=255)
                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaa                                                   >  2:319451/1‑139 (MQ=255)
                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaa                                                   >  1:362157/1‑139 (MQ=255)
                                                                                aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                <  2:865644/139‑1 (MQ=255)
                                                                                  cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                              <  1:352116/139‑1 (MQ=255)
                                                                                  cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                              <  2:261276/139‑1 (MQ=255)
                                                                                      ggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACctt                                          <  1:239929/139‑1 (MQ=255)
                                                                                                 aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                              >  1:8930/1‑124 (MQ=255)
                                                                                                 aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                              <  2:8930/124‑1 (MQ=255)
                                                                                                  aCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                            >  1:32192/1‑45 (MQ=255)
                                                                                                  aCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                            <  2:32192/45‑1 (MQ=255)
                                                                                                     tGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAc                           >  2:961305/1‑139 (MQ=255)
                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                          >  2:1093963/1‑139 (MQ=255)
                                                                                                        aGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGt                                                                               <  2:268921/84‑1 (MQ=255)
                                                                                                        aGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGt                                                                               >  1:268921/1‑84 (MQ=255)
                                                                                                         gTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGcc                       >  1:712482/1‑139 (MQ=255)
                                                                                                          ttCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCt                      <  2:266182/139‑1 (MQ=255)
                                                                                                            cAGCCCACCGGGATGAGATAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACTTTCTTATAGAACCCAACCGCCTGc                    <  1:528117/139‑1 (MQ=255)
                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCAcc                                                                          <  1:354789/84‑1 (MQ=255)
                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCAcc                                                                          >  2:354789/1‑84 (MQ=255)
                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                    >  2:332758/1‑90 (MQ=255)
                                                                                                             aGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                    <  1:332758/90‑1 (MQ=255)
                                                                                                                  aCCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                 <  1:378914/56‑1 (MQ=255)
                                                                                                                  aCCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                 >  2:378914/1‑56 (MQ=255)
                                                                                                                    cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg            >  1:1067434/1‑139 (MQ=255)
                                                                                                                    cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg            <  2:235078/139‑1 (MQ=255)
                                                                                                                    cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg            <  1:397112/139‑1 (MQ=255)
                                                                                                                            gaAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTaa    <  2:614596/139‑1 (MQ=255)
                                                                                                                              aaaaaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg  <  1:961305/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACG  >  NC_000913/4213545‑4213809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑