Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,809	C→T	*68* (TAG→TAA)	yjbS ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,809	0	C	T	100.0%	152.3 / NA	48	*68* (TAG→TAA)	yjbS	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/23);  total (25/23)

GTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTA  >  NC_000913/4268677‑4268944                                                                                                                                     |                                                                                                                                        gTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATc                                                                                                                                   >  1:151130/1‑139 (MQ=255)      aCCTTATATTGATGACCAATAACAAATAAAAAGGTCCTGTAATGAGAATATTCAACTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATGTACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATaa                                                                                                                              >  2:1022322/1‑139 (MQ=255)             atTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTg                                                                                                                       <  2:151130/139‑1 (MQ=255)               tGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGaa                                                                                                                     <  2:42695/139‑1 (MQ=255)                  ttACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGaaaaa                                                                                                                  >  2:633830/1‑139 (MQ=255)                      cAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACttt                                                                                                              >  2:284022/1‑139 (MQ=255)                              aaTAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCaa                                                                                                      >  2:84009/1‑139 (MQ=255)                              aaTAAAAAGGTCCTGTAATGACAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCaa                                                                                                      <  1:652556/139‑1 (MQ=255)                                tAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATa                                                                                                    <  1:284022/139‑1 (MQ=255)                                    aaGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAAc                                                                                                <  2:96802/139‑1 (MQ=255)                                         ccTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                           >  2:146129/1‑139 (MQ=255)                                         ccTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                           <  1:633830/139‑1 (MQ=255)                                                  agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTtcttct                                                                                  >  1:10138/1‑139 (MQ=255)                                                    aataatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTcc                                                                                >  2:562244/1‑139 (MQ=255)                                                              tttAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGa                                                                                            <  2:780720/117‑1 (MQ=255)                                                              tttAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGa                                                                                            >  1:780720/1‑117 (MQ=255)                                                                       ctcGGCAAAACAGCATAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTATTCTCCAGGTCTAAAAAATCTTTTg                                                             >  1:560565/1‑139 (MQ=255)                                                                       ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                             >  2:71675/1‑139 (MQ=255)                                                                         cGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCa                                                           <  2:12654/139‑1 (MQ=255)                                                                                 cAGCAAAGCGTGCAAATATACATAAATTCTTTCTTACGCCTAATTTTTTTAATTCAATTAATAAATGCGTTTTAATCTTTACCTGCCATAGCACTTGATACCTCTTTCCCATGTCTAAAAAATCTTTTGCAATTTTTaa                                                   <  2:560565/139‑1 (MQ=255)                                                                                                   taCATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATaa                                 >  2:119123/1‑139 (MQ=255)                                                                                                     cATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                 >  2:492785/1‑121 (MQ=255)                                                                                                     cATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                 <  1:492785/121‑1 (MQ=255)                                                                                                        aaaGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGa                                                                                                  >  1:786899/1‑69 (MQ=255)                                                                                                        aaaGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGa                                                                                                  <  2:786899/69‑1 (MQ=255)                                                                                                          aGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGa                                                                                            >  2:155521/1‑73 (MQ=255)                                                                                                          aGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGa                                                                                            <  1:155521/73‑1 (MQ=255)                                                                                                          aGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAg                                                                              >  2:279936/1‑87 (MQ=255)                                                                                                          aGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAg                                                                              <  1:279936/87‑1 (MQ=255)                                                                                                          aGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGaaa                          >  2:130867/1‑139 (MQ=255)                                                                                                            tctttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATc                        >  1:606991/1‑139 (MQ=255)                                                                                                              tttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                                 >  2:739417/1‑80 (MQ=255)                                                                                                              tttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                                 <  1:739417/80‑1 (MQ=255)                                                                                                              tttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCtt                      >  2:525796/1‑139 (MQ=255)                                                                                                                    aCCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCAt                <  1:146129/139‑1 (MQ=255)                                                                                                                          gATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTcc                                                                                <  1:1015068/69‑1 (MQ=255)                                                                                                                          gATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTcc                                                                                >  2:1015068/1‑69 (MQ=255)                                                                                                                          gATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTACAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAgaga          >  2:794693/1‑139 (MQ=255)                                                                                                                          gATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAgaga          <  1:119123/139‑1 (MQ=255)                                                                                                                           aTTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCa                                                                               >  1:396668/1‑69 (MQ=255)                                                                                                                           aTTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCa                                                                               <  2:396668/69‑1 (MQ=255)                                                                                                                           aTTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGat         >  2:648146/1‑139 (MQ=255)                                                                                                                           aTTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGat         <  1:525796/139‑1 (MQ=255)                                                                                                                             tttGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGatat       <  1:648146/139‑1 (MQ=255)                                                                                                                             tttGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGatat       <  2:1084170/139‑1 (MQ=255)                                                                                                                                gTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                 >  1:227279/1‑94 (MQ=255)                                                                                                                                gTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                 <  2:227279/94‑1 (MQ=255)                                                                                                                                  tAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTtta  <  2:838175/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTA  >  NC_000913/4268677‑4268944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑