Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,302,240 A→G I280T (ATC→ACC)  mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,302,2400AG100.0% 175.9 / NA 49I280T (ATC→ACC) mdtOmembrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

CCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCA  >  NC_000913/4302111‑4302361
                                                                                                                                 |                                                                                                                         
cccAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAg                                                                                                                  <  1:470509/139‑1 (MQ=255)
             acGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  1:1109464/1‑139 (MQ=255)
              cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    >  2:605932/1‑139 (MQ=255)
               gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGa                                                                                                   >  1:1112950/1‑139 (MQ=255)
                             ctcCAGGTTACATTCCCGTGTCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTAAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGcc                                                                                     <  2:229419/139‑1 (MQ=255)
                             ctcCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGcc                                                                                     >  1:160185/1‑139 (MQ=255)
                               ccAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTg                                                                                   >  2:807437/1‑139 (MQ=255)
                                  ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                <  1:487418/139‑1 (MQ=255)
                                  ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                >  2:231901/1‑139 (MQ=255)
                                     tACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATc                                                                             >  1:404775/1‑139 (MQ=255)
                                      aCATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCa                                                                            <  2:404775/139‑1 (MQ=255)
                                              gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAaga                                                                    >  1:1076098/1‑139 (MQ=255)
                                                  cgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTg                                                                >  2:455110/1‑139 (MQ=255)
                                                              actttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGa                                                    >  1:184508/1‑139 (MQ=255)
                                                                 ttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  1:176845/1‑87 (MQ=255)
                                                                 ttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     <  2:176845/87‑1 (MQ=255)
                                                                       gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaaa                                                                      <  1:76926/112‑1 (MQ=255)
                                                                       gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaaa                                                                      >  2:76926/1‑112 (MQ=255)
                                                                         tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         <  2:1112950/139‑1 (MQ=255)
                                                                         tCCGCAAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGACTGAGAAt                                                                              >  1:145311/1‑102 (MQ=255)
                                                                         tCCGCAAGTCGCTTTGCCAGCACTGAACTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTAGTCGGAATTCAATAATTGCCTGAGAAt                                                                              <  2:145311/102‑1 (MQ=255)
                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                            >  1:628014/1‑135 (MQ=255)
                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                            <  2:628014/135‑1 (MQ=255)
                                                                          ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                        <  1:455110/139‑1 (MQ=255)
                                                                           cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                       <  2:131947/139‑1 (MQ=255)
                                                                             ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCCGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                          >  1:743994/1‑86 (MQ=255)
                                                                             ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                          <  2:743994/86‑1 (MQ=255)
                                                                               aGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   <  2:1076098/139‑1 (MQ=255)
                                                                                gTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGaa                                                                                                                   <  2:1112964/58‑1 (MQ=255)
                                                                                gTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGaa                                                                                                                   >  1:1112964/1‑58 (MQ=255)
                                                                                  cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     <  1:238283/70‑1 (MQ=255)
                                                                                  cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                     >  2:238283/1‑70 (MQ=255)
                                                                                   gCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAg                               <  2:160185/139‑1 (MQ=255)
                                                                                                  aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGACGGAATTCAATAATTGCCTGAGAAGCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACg                >  1:681026/1‑139 (MQ=255)
                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       >  1:257628/1‑51 (MQ=255)
                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       <  2:257628/51‑1 (MQ=255)
                                                                                                   ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc               <  2:1109464/139‑1 (MQ=255)
                                                                                                     ttCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAg             <  2:185879/139‑1 (MQ=255)
                                                                                                       cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    <  2:383784/50‑1 (MQ=255)
                                                                                                       cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                    >  1:383784/1‑50 (MQ=255)
                                                                                                               gCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       >  2:577678/1‑39 (MQ=255)
                                                                                                               gCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                       <  1:577678/39‑1 (MQ=255)
                                                                                                                cATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGCATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccacca  <  2:681026/139‑1 (MQ=255)
                                                                                                                       cAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         <  1:862765/93‑1 (MQ=255)
                                                                                                                       cAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                         >  2:862765/1‑93 (MQ=255)
                                                                                                                        aGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   <  2:295785/98‑1 (MQ=255)
                                                                                                                        aGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                   >  1:295785/1‑98 (MQ=255)
                                                                                                                             gttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAg                                               >  2:143785/1‑81 (MQ=255)
                                                                                                                             gttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAg                                               <  1:143785/81‑1 (MQ=255)
                                                                                                                                 |                                                                                                                         
CCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCA  >  NC_000913/4302111‑4302361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑