Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,326,034 | A→G | intergenic (‑293/+365) | yjdN ← / ← yjdM | metalloprotein superfamily protein/zinc‑ribbon family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,326,034 | 0 | A | G | 94.4% | 37.8 / ‑4.1 | 18 | intergenic (‑293/+365) | yjdN/yjdM | metalloprotein superfamily protein/zinc‑ribbon family protein |
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (6/11); total (7/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 |
CCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCA > NC_000913/4325907‑4326145 | ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCa < 1:306688/139‑1 (MQ=255) gcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATg < 2:151921/139‑1 (MQ=37) cAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgc > 2:1043979/1‑139 (MQ=39) cAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCCTCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTAt < 2:788070/139‑1 (MQ=255) aCCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATCCGGCCTa > 1:788070/1‑139 (MQ=18) gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATg < 2:532747/139‑1 (MQ=14) cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGt > 1:573370/1‑139 (MQ=17) cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGa < 2:573370/139‑1 (MQ=17) ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg > 1:616118/1‑121 (MQ=17) ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg < 2:616118/121‑1 (MQ=18) gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt < 1:574579/139‑1 (MQ=18) gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAt > 2:574579/1‑139 (MQ=17) aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg > 1:802165/1‑139 (MQ=17) aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg < 2:802165/139‑1 (MQ=18) aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg < 2:769545/139‑1 (MQ=17) ccTGATAAGACGCGCAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa < 2:644191/139‑1 (MQ=255) cAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg < 2:372141/76‑1 (MQ=255) cAAGCGTCGCATCGGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCggg > 1:372141/1‑76 (MQ=19) | CCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCA > NC_000913/4325907‑4326145 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |