Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	144.1 / NA	40	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

TCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTT  >  NC_000913/4389805‑4390069                                                                                                                                  |                                                                                                                                        tCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                                <  1:904216/139‑1 (MQ=255)          cgttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTa                                                                                                                       >  1:341304/1‑139 (MQ=255)           gttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTaa                                                                                                                      <  1:110053/139‑1 (MQ=255)                gggCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                                 <  1:772489/139‑1 (MQ=255)                gggCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                                 <  2:341304/139‑1 (MQ=255)                gggCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACAGTTGCACGGGATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                                 <  2:684955/139‑1 (MQ=255)                   cAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTcac                                                                                                              <  1:759701/139‑1 (MQ=255)                      aCAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACaaa                                                                                                           >  1:278591/1‑139 (MQ=255)                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGTACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                        >  2:942661/1‑102 (MQ=255)                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGTACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGt                                                                                                                        <  1:942661/102‑1 (MQ=255)                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   <  1:372780/91‑1 (MQ=255)                                              tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   >  2:372780/1‑91 (MQ=255)                                                    ggTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAg                                                                             <  2:278591/139‑1 (MQ=255)                                                        aaCGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   <  2:865363/81‑1 (MQ=255)                                                        aaCGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   >  1:865363/1‑81 (MQ=255)                                                            gagaAGAGCTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGt                                                                                                   >  1:504472/1‑109 (MQ=255)                                                            gagaAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGt                                                                                                   <  2:504472/109‑1 (MQ=255)                                                                agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGAt                                                                           >  1:913113/1‑129 (MQ=255)                                                                agagATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGAt                                                                           <  2:913113/129‑1 (MQ=255)                                                                                       atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGt                                          >  1:804130/1‑139 (MQ=255)                                                                                       atcTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGt                                          >  1:280094/1‑139 (MQ=255)                                                                                          tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   <  1:747225/47‑1 (MQ=255)                                                                                          tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                                   >  2:747225/1‑47 (MQ=255)                                                                                                  aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgggg                                                                                                                              <  2:986417/44‑1 (MQ=255)                                                                                                  aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgggg                                                                                                                              >  1:986417/1‑44 (MQ=255)                                                                                                    tACCGTTGCACGGCATGTTTACGCGGTGGTGGTCAtgcg                                                                                                                                 <  1:754974/39‑1 (MQ=255)                                                                                                    tACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcgcg                                                                                                                                 >  2:754974/1‑39 (MQ=38)                                                                                                          tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                                   >  1:561286/1‑79 (MQ=255)                                                                                                          tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                                   <  2:561286/79‑1 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGt                                                                        >  1:15476/1‑88 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGt                                                                        <  2:15476/88‑1 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAg                                           <  2:286947/117‑1 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAg                                           >  1:286947/1‑117 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGtt                                         >  1:494143/1‑119 (MQ=255)                                                                                                            cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGtt                                         <  2:494143/119‑1 (MQ=255)                                                                                                                 cATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATc                                                                               <  2:1025769/76‑1 (MQ=255)                                                                                                                 cATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATc                                                                               >  1:1025769/1‑76 (MQ=255)                                                                                                                    gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTAtct             >  1:834800/1‑139 (MQ=255)                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGa      <  2:804130/139‑1 (MQ=255)                                                                                                                           cgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGa      <  2:280094/139‑1 (MQ=255)                                                                                                                              tggtggTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                      <  2:809863/56‑1 (MQ=255)                                                                                                                              tggtggTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                                      >  1:809863/1‑56 (MQ=255)                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCtt                                                      <  1:21344/87‑1 (MQ=255)                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGAttt                  >  2:440886/1‑123 (MQ=255)                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGAttt                  <  1:440886/123‑1 (MQ=255)                                                                                                                               ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGAtttt  >  1:300326/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        TCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTT  >  NC_000913/4389805‑4390069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑