Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,849	A→G	T264T (ACA→ACG)	yjhB →	putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,849	0	A	G	100.0%	226.0 / NA	63	T264T (ACA→ACG)	yjhB	putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

TTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTC  >  NC_000913/4504719‑4504979                                                                                                                                   |                                                                                                                                   ttttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAgg                                                                                                                            >  2:513839/1‑139 (MQ=255) ttttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAgg                                                                                                                            >  1:383668/1‑139 (MQ=255)   ttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                          >  2:308657/1‑139 (MQ=255)   ttCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTa                                                                                                                          >  1:318575/1‑139 (MQ=255)     ccTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACa                                                                                                                        <  1:1030964/139‑1 (MQ=255)      cTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACaa                                                                                                                       <  1:308657/139‑1 (MQ=255)                     cTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttg                                                                                                        <  1:900469/139‑1 (MQ=255)                       aTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                      <  2:91120/139‑1 (MQ=255)                        tttGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                      >  1:946320/1‑138 (MQ=255)                        tttGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgtt                                                                                                      <  2:946320/138‑1 (MQ=255)                          tGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGt                                                                                                   <  1:1004694/139‑1 (MQ=255)                                  aCTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                           >  1:71411/1‑139 (MQ=255)                                       ccGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                                      >  1:1039161/1‑139 (MQ=255)                                              aCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTaaaaaa                                                                               <  2:71411/139‑1 (MQ=255)                                                  actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAt                                                                                                <  2:574575/118‑1 (MQ=255)                                                  actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAt                                                                                                >  1:574575/1‑118 (MQ=255)                                                          cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGt                                                                   <  2:383668/139‑1 (MQ=255)                                                           ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                  <  1:849845/139‑1 (MQ=255)                                                              ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                  >  1:1115359/1‑136 (MQ=255)                                                              ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                  <  2:1115359/136‑1 (MQ=255)                                                                   tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                            >  1:902443/1‑105 (MQ=255)                                                                   tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                            <  2:902443/105‑1 (MQ=255)                                                                            aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                       <  2:167960/85‑1 (MQ=255)                                                                            aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                       >  1:167960/1‑85 (MQ=255)                                                                               gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGt                                                               >  2:459972/1‑122 (MQ=255)                                                                               gAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGt                                                               <  1:459972/122‑1 (MQ=255)                                                                                   tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGa                                                                                                                                >  1:982840/1‑53 (MQ=255)                                                                                   tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGa                                                                                                                                <  2:982840/53‑1 (MQ=255)                                                                                     aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTc                                                    <  2:310959/127‑1 (MQ=255)                                                                                     aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTc                                                    >  1:310959/1‑127 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAt                                                                                                <  2:327558/78‑1 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGAt                                                                                                >  1:327558/1‑78 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACtt                                                     <  1:315316/121‑1 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACtt                                                     >  2:315316/1‑121 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCGTTTATTTTCCTTTGTc                                   >  1:754804/1‑139 (MQ=255)                                                                                          gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTc                                   >  1:315945/1‑139 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                       >  2:3435/1‑69 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTttgt                                                                                                       <  1:3435/69‑1 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatttatt                                             <  1:659256/127‑1 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatttatt                                             >  2:659256/1‑127 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                 >  2:374260/1‑139 (MQ=255)                                                                                            ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                 >  2:1076418/1‑139 (MQ=255)                                                                                             gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctc                                >  2:390054/1‑139 (MQ=255)                                                                                                aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                            >  2:440961/1‑76 (MQ=255)                                                                                                aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGa                                                                                            <  1:440961/76‑1 (MQ=255)                                                                                                aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                             >  1:445721/1‑139 (MQ=255)                                                                                                     aaCTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                           >  1:358886/1‑72 (MQ=255)                                                                                                     aaCTCTGATGACAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAt                                                                                           <  2:358886/72‑1 (MQ=255)                                                                                                            atgaCAATAGCTGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                 >  2:644793/1‑139 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                                      >  1:816564/1‑70 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGgtg                                                                                      <  2:816564/70‑1 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                              >  2:542860/1‑126 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                              <  1:542860/126‑1 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                 >  1:429401/1‑139 (MQ=255)                                                                                                            atgaCAATAGCAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCtg                 >  1:554146/1‑139 (MQ=255)                                                                                                                      cAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                              >  2:472039/1‑116 (MQ=255)                                                                                                                      cAGGTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                              <  1:472039/116‑1 (MQ=255)                                                                                                                        ggTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt     <  2:429401/139‑1 (MQ=255)                                                                                                                        ggTTTAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt     <  1:644793/139‑1 (MQ=255)                                                                                                                        ggTTTAGGAACGCCGACAGGTATAATATTTTTTGGTGTTGTTGGTTATTAGATTGGTGTCAAAAAAGCCTTTGTAGTCGGTCTAATTACTTCATTTATTTTCCGTTATCCTCTTTTTTTTATTTCTGTGAAAAActctt     <  2:599560/139‑1 (MQ=255)                                                                                                                           ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                  >  2:408027/1‑75 (MQ=255)                                                                                                                           ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTc                                                                  <  1:408027/75‑1 (MQ=255)                                                                                                                           ttAGGAACGCTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc  >  2:429666/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   TTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTC  >  NC_000913/4504719‑4504979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑