Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,188	A→G	intergenic (+55/+502)	yjhV → / ← fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,188	0	A	G	100.0%	206.0 / NA	57	intergenic (+55/+502)	yjhV/fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/26);  total (31/26)

GCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCT  >  NC_000913/4510053‑4510315                                                                                                                                        |                                                                                                                                gCCAGCTTCGGTAAGATGGGTGAGACACCGACTCATGAAGATATCATTGATCACAATCGATCCATCAACTGGCCTGTATAACGTGGCAGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCtt                                                                                                                              >  2:741560/1‑139 (MQ=255)                         acacCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGaaa                                                                                                      <  2:1037315/139‑1 (MQ=255)                                          tatCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCttt                                                                                                                             <  1:532990/99‑1 (MQ=255)                                          tatCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCttt                                                                                                                             >  2:532990/1‑99 (MQ=255)                                           atCATTGATAACAATCGAACCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGAACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTACGt                                                                                    >  2:617523/1‑139 (MQ=255)                                                    aaCAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAg                                                                                             <  1:131051/121‑1 (MQ=255)                                                    aaCAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAg                                                                                             >  2:131051/1‑121 (MQ=255)                                                        aTCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCtt                                                                       <  2:536353/139‑1 (MQ=255)                                                                     cTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGtt                                                           >  2:455681/1‑138 (MQ=255)                                                                     cTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGtt                                                           <  1:455681/138‑1 (MQ=255)                                                                       ggCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGc                                                        >  2:934354/1‑139 (MQ=255)                                                                         ccTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                      <  2:1041796/139‑1 (MQ=255)                                                                          cTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGa                                                                                                                >  2:284582/1‑80 (MQ=255)                                                                          cTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGa                                                                                                                <  1:284582/80‑1 (MQ=255)                                                                           tGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                      >  2:130470/1‑137 (MQ=255)                                                                           tGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                      <  1:130470/137‑1 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                        <  1:126291/91‑1 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                        >  2:126291/1‑91 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGtt                                                                                   <  2:451699/96‑1 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGtt                                                                                   >  1:451699/1‑96 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGt                                                                            >  1:133054/1‑103 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGt                                                                            <  2:133054/103‑1 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGAttt                                                                <  2:391114/115‑1 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGAttt                                                                >  1:391114/1‑115 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                        >  2:549158/1‑139 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                        >  1:267636/1‑139 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                        >  2:1038614/1‑139 (MQ=255)                                                                                       cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                        >  1:369383/1‑139 (MQ=255)                                                                                               gATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCaa                                <  1:549158/139‑1 (MQ=255)                                                                                                          cTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGAGTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCaaa                               <  2:829057/129‑1 (MQ=255)                                                                                                          cTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGAGTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCaaa                               >  1:829057/1‑129 (MQ=255)                                                                                                               cctacctaAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGt                                                                                    <  1:420787/71‑1 (MQ=255)                                                                                                               cctacctaAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGt                                                                                    >  2:420787/1‑71 (MQ=255)                                                                                                                          agtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAg                                                                                             >  2:216847/1‑51 (MQ=255)                                                                                                                          agtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAg                                                                                             <  1:216847/51‑1 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                      >  1:929314/1‑88 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGt                                                      <  2:929314/88‑1 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                               >  2:309952/1‑95 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                               <  1:309952/95‑1 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                          >  2:551672/1‑100 (MQ=255)                                                                                                                            tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                          <  1:551672/100‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAGCTGGATTAGCAATTCCGTTCCATgg                                                                             <  2:770125/64‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                        >  1:720079/1‑53 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                        <  2:720079/53‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCt                                                                        >  1:863060/1‑69 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCt                                                                        <  2:863060/69‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACg                                                             >  2:762590/1‑80 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACg                                                             <  1:762590/80‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCa                                 >  2:115724/1‑108 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCa                                 <  1:115724/108‑1 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt  >  1:595795/1‑139 (MQ=255)                                                                                                                             agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt  >  2:962166/1‑139 (MQ=255)                                                                                                                             agCAAACGCACGCTTTCTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATgg                                                                             >  1:770125/1‑64 (MQ=255)                                                                                                                              gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                               <  1:850461/93‑1 (MQ=255)                                                                                                                              gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                               >  2:850461/1‑93 (MQ=255)                                                                                                                                   cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCaa                                                                                          >  1:236067/1‑45 (MQ=255)                                                                                                                                   cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCaa                                                                                          <  2:236067/45‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCT  >  NC_000913/4510053‑4510315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑