Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,604,202 G→A *242* (TAG→TAA) 
E15K (GAA→AAA) 		 yjjQ →
bglJ →		 putative transcriptional regulator
bgl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,2020GA100.0% 274.8 / NA 85*242* (TAG→TAA) 
E15K (GAA→AAA) 		yjjQ
bglJ		putative transcriptional regulator
bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/47);  total (38/47)

ACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC  >  NC_000913/4604072‑4604332
                                                                                                                                  |                                                                                                                                  
acaGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACGTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGc                                                                                                                            <  1:535002/139‑1 (MQ=255)
  aGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGt                                                                                                                          <  1:243698/139‑1 (MQ=255)
   gCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACATAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  2:926832/139‑1 (MQ=255)
   gCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  2:1109046/139‑1 (MQ=255)
   gCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  2:690475/139‑1 (MQ=255)
       cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt                                                                                                                       >  1:864437/1‑137 (MQ=255)
       cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt                                                                                                                       <  2:864437/137‑1 (MQ=255)
       cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAAAGAAATGTCGCACTCAAAAAAAAATGAGTCATGa                                                                                                                     >  2:535002/1‑139 (MQ=255)
         cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGc                                                                                                                            <  2:324249/130‑1 (MQ=255)
         cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGc                                                                                                                            >  1:324249/1‑130 (MQ=255)
         cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                                                                   <  1:490666/139‑1 (MQ=255)
         cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTGATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                                                                   <  2:796347/139‑1 (MQ=255)
             aaTGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               <  2:609775/139‑1 (MQ=255)
               tGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACCTGCATGCTGTTTATGGAGAGGCCGCAGAATGGAACACAGCCGAGTTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc                                                                                                             <  2:488204/139‑1 (MQ=255)
                    gCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTAt                                                                                                        <  2:309660/139‑1 (MQ=255)
                      aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc                                                                                                             <  1:1023001/132‑1 (MQ=255)
                      aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc                                                                                                             >  2:1023001/1‑132 (MQ=255)
                      aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      >  2:96023/1‑139 (MQ=255)
                      aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      <  2:604486/139‑1 (MQ=255)
                      aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACACATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      >  2:553001/1‑139 (MQ=255)
                        ggATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTgag                                                                                                    >  1:301478/1‑139 (MQ=255)
                        ggATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTgag                                                                                                    >  1:231504/1‑139 (MQ=255)
                            aaaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGtt                                                                                                <  2:301478/139‑1 (MQ=255)
                                 gcgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTc                                                                                           >  1:303176/1‑139 (MQ=255)
                                  cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCa                                                                                          >  2:778749/1‑139 (MQ=255)
                                  cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCa                                                                                          >  1:748790/1‑139 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAGCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                                                        >  1:311433/1‑139 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         >  2:613781/1‑106 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  1:613781/106‑1 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                                                                   >  1:539662/1‑112 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                                                                   <  2:539662/112‑1 (MQ=255)
                                    cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                                                        >  1:364266/1‑139 (MQ=255)
                                         ggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTAt                                                                                                              <  1:319088/112‑1 (MQ=255)
                                         ggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTAt                                                                                                              >  2:319088/1‑112 (MQ=255)
                                           tGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTg                                                                                 <  1:778749/139‑1 (MQ=255)
                                               agagAACTTTACTTGCCTTCTGTGTATGGAGATGCCGCAGACTGGAACACAGCAGAATTAAGAAGATATATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTGTATTCAGAAAGTTTGCggg                                                                             <  1:553001/139‑1 (MQ=255)
                                                     cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         >  1:130274/1‑89 (MQ=255)
                                                     cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  2:130274/89‑1 (MQ=255)
                                                     cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             <  1:131043/133‑1 (MQ=255)
                                                     cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             >  2:131043/1‑133 (MQ=255)
                                                       taacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  1:893252/87‑1 (MQ=255)
                                                       taacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         >  2:893252/1‑87 (MQ=255)
                                                            tGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGATTATTCAGAAAGTTTGCGGGTAACCCTTATAAg                                                                <  2:231504/139‑1 (MQ=255)
                                                             gCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGc                                                               >  2:86245/1‑139 (MQ=255)
                                                               cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  2:748790/139‑1 (MQ=255)
                                                               cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  1:446744/139‑1 (MQ=255)
                                                                ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  1:1050231/139‑1 (MQ=255)
                                                                ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  2:364266/139‑1 (MQ=255)
                                                                ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  2:303176/139‑1 (MQ=255)
                                                                         tGGAGATGCCGCAGAATGGAGCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCtata                                                   <  2:311433/139‑1 (MQ=255)
                                                                             gaTGCCGTAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCta                                                     >  1:932898/1‑133 (MQ=255)
                                                                             gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  1:1056096/125‑1 (MQ=255)
                                                                             gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             >  2:1056096/1‑125 (MQ=255)
                                                                             gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCta                                                     <  2:932898/133‑1 (MQ=255)
                                                                             gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAg                                               >  1:610966/1‑139 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt                                                                                                                       >  2:61823/1‑65 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt                                                                                                                       <  1:61823/65‑1 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                                                                    >  2:1104541/1‑68 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                                                                    <  1:1104541/68‑1 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             <  1:218953/107‑1 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             >  2:218953/1‑107 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATAc                                                        >  2:556012/1‑128 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATAc                                                        <  1:556012/128‑1 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTc                                             >  2:883222/1‑139 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTc                                             >  2:639876/1‑139 (MQ=255)
                                                                               tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTAATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAGCCTTATAAGCTCCATACCTATACCAGTc                                             >  2:1045085/1‑139 (MQ=255)
                                                                                     aGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGt                                       <  1:96023/139‑1 (MQ=255)
                                                                                        aTGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCAt                                    >  2:1034732/1‑139 (MQ=255)
                                                                                          ggAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCAttt                                  >  2:659731/1‑139 (MQ=255)
                                                                                            aaCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCa                                <  1:639876/139‑1 (MQ=255)
                                                                                            aaCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCa                                <  1:86245/139‑1 (MQ=255)
                                                                                                 aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  1:554320/105‑1 (MQ=255)
                                                                                                 aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             >  2:554320/1‑105 (MQ=255)
                                                                                                 aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATg                           <  2:610966/139‑1 (MQ=255)
                                                                                                   ccGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg                                                                                                                      >  2:372527/1‑46 (MQ=255)
                                                                                                   ccGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg                                                                                                                      <  1:372527/46‑1 (MQ=255)
                                                                                                     gAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCAt                       <  1:659731/139‑1 (MQ=255)
                                                                                                       aTTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGGTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGt                     <  2:795084/139‑1 (MQ=255)
                                                                                                         tAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTcg                   <  1:883222/139‑1 (MQ=255)
                                                                                                             aagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                                                          >  2:450535/1‑80 (MQ=255)
                                                                                                             aagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                                                          <  1:450535/80‑1 (MQ=255)
                                                                                                                agaAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGtt                                                                                   >  2:456460/1‑68 (MQ=255)
                                                                                                                agaAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGtt                                                                                   <  1:456460/68‑1 (MQ=255)
                                                                                                                          cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggc  >  1:484294/1‑139 (MQ=255)
                                                                                                                          cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggc  >  1:482405/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                  
ACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC  >  NC_000913/4604072‑4604332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑