Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,816	T→C	V468A (GTT→GCT)	prfC →	peptide chain release factor RF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,816	0	T	C	100.0%	172.2 / NA	47	V468A (GTT→GCT)	prfC	peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

GGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGT  >  NC_000913/4610685‑4610939                                                                                                                                    |                                                                                                                            ggTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACt                                                                                                                      <  2:211451/139‑1 (MQ=255)             ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                >  2:361061/1‑133 (MQ=255)             ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                <  1:361061/133‑1 (MQ=255)             ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                          >  2:316457/1‑139 (MQ=255)             ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                          >  2:192573/1‑139 (MQ=255)             ccGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTa                                                                                                          >  1:491867/1‑139 (MQ=255)               gTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGa                                                                                                        <  1:572756/139‑1 (MQ=255)                          aacaacGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGa                                                                                                        <  1:705954/128‑1 (MQ=255)                          aacaacGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGa                                                                                                        >  2:705954/1‑128 (MQ=255)                                        cGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                               <  1:411515/139‑1 (MQ=255)                                         gTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAag                                                                              <  1:316457/139‑1 (MQ=255)                                              tGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACt                                                                                                                      <  1:1085510/94‑1 (MQ=255)                                              tGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACt                                                                                                                      >  2:1085510/1‑94 (MQ=255)                                              tGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTc                                                                         <  1:192573/139‑1 (MQ=255)                                                       tgtgCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAg                                                                >  1:929058/1‑139 (MQ=255)                                                                gTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGc                                                       >  1:453597/1‑139 (MQ=255)                                                                         ggtggtAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGgcg                                              <  2:374017/139‑1 (MQ=255)                                                                                     ccTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcggc                                  >  1:965568/1‑139 (MQ=255)                                                                                      cTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGgcggcg                                 >  1:243634/1‑139 (MQ=255)                                                                                       tGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                <  1:388894/59‑1 (MQ=255)                                                                                       tGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGc                                                                                                                >  2:388894/1‑59 (MQ=255)                                                                                       tGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                               <  1:872995/92‑1 (MQ=255)                                                                                       tGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                               >  2:872995/1‑92 (MQ=255)                                                                                         aaGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  >  2:118076/1‑55 (MQ=255)                                                                                         aaGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  <  1:118076/55‑1 (MQ=255)                                                                                           gagCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAAc                            <  2:929058/139‑1 (MQ=255)                                                                                              cGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  <  1:878407/50‑1 (MQ=255)                                                                                              cGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  >  2:878407/1‑50 (MQ=255)                                                                                              cGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                               >  2:254220/1‑85 (MQ=255)                                                                                              cGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGaa                                                                               <  1:254220/85‑1 (MQ=255)                                                                                                aaTACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggc                                     <  1:936411/125‑1 (MQ=255)                                                                                                aaTACAACGTTGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggc                                     >  2:936411/1‑125 (MQ=255)                                                                                                          tGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  <  1:377815/38‑1 (MQ=255)                                                                                                          tGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGccc                                                                                                                  >  2:377815/1‑38 (MQ=38)                                                                                                          tGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCt             >  2:332854/1‑139 (MQ=255)                                                                                                          tGAAGCAGTGTATGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCt             >  1:93951/1‑139 (MQ=255)                                                                                                                     aTGAGTCAGTCAACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGt  <  2:965568/139‑1 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAAc                                                   <  2:708273/83‑1 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAAc                                                   >  1:708273/1‑83 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                 >  2:336001/1‑117 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACAt                 <  1:336001/117‑1 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCACTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATa                              <  2:1073569/104‑1 (MQ=255)                                                                                                                            agtcaACGCTGCCACTGCCCACTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATa                              >  1:1073569/1‑104 (MQ=255)                                                                                                                              tcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcgg                                   <  1:567364/97‑1 (MQ=255)                                                                                                                              tcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcgg                                   >  2:567364/1‑97 (MQ=255)                                                                                                                              tcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcgg                                   <  1:998717/97‑1 (MQ=255)                                                                                                                              tcaACGCTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATggcgg                                   >  2:998717/1‑97 (MQ=255)                                                                                                                                    |                                                                                                                            GGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGT  >  NC_000913/4610685‑4610939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑